Histology Stains

Mucicarmine

What stain is this?

Alcian Blue pH 2.5 (and PAS)

What stain is this?

Oil Red O

What stain is this?

Gordon and Sweets

What stain is this?

Brown and Brenn

What stain is this?

PAS

What stain is this?

PAS with diastase

What stain is this?

Grcott's methenamine silver

What stain is this?

Verhoff Van Gieson

What stain is this?

Hematoxylin and Eosin (H&E)

What stain is this?

Weigert's Van Gieson

What stain is this?

Masson Trichrome

What stain is this?

Hall's Bile stain

What stain is this?

Immunohistochemistry (IHC)

What stain is this?

Nuclei = blue/purple

RBC = pink/orange

Muscle = dark pink/red

Collagen/cytoplasm = light pink

What tissue elements are stained in H&E?

Nuclei = black

Elastic fibres = blue/black

Collagen = red

Other tissue elements (muscle/RBC) = yellow

What tissue elements are stained by Weigert's Van Gieson?

Nuclei = blue/black

RBC, muscle, cytoplasm = red

Collagen, cartilage, mucin = blue/green

What tissue elements does Masson trichrome stain?

Elastic fibres = black

Nuclei = blue/black

Collagen = red

Other tissue elements = yellow

What tissue elements are stained in Verhoeff Van Gieson?

Reticulin = black

Other tissue elements = counterstain (green/red)

What tissue elements are stained in Gordon and Sweets?

Glycogen, muco & glycoproteins, glycosaminocans = bright rose

Background = purple

What tissue elements are stained by PAS?

Mucosubstances (carboxylated and sulphated) = dark blue

Background = red/pink

What tissue elements are stained in Alcian blue pH 2.5?

Mucin, capsule of cryptococcus = deep rose/red

Nuclei = black

Background = yellow

What tissue elements are stained in Mucicarmine?

Gram + bacteria, fibrin, fungi, paneth cells, keratolyalin, keratin = blue

Gram - bacteria = red

Other/background = yellow

What elements are stained in the Brown and Brenn?

Nuclei = pink

Melanin and argentaffin granules = black

What elements are stained in the Masson Fontana stain?

Fungi (cell walls) = black

Mucin = taupe/dark grey

Background = green

What tissue elements are stained in Grocott's methenamine silver?

Lipids = red

Other/background = counterstain

What elements are stained in Oil Red O?

Bile/bilirubin = emerald/olive green

Background = yellow

What elements are stained in Hall's Bile?

Antigen = red/brown

Background = blue

What elements are stained in Immunohistochemical staining?

Routine staining

What is H&E used for?

Collagen

What is WVG used for?

Connective tissue

What is Masson Trichrome used for?

Elastin

What is Verhoeff Van Gieson used for?

Reticulin

What is Gordon and sweets used for?

Glycogen

What is PAS used for?

Mucins

What is Mucicarmine used for?

Bacteria

What is Brown and Brenn used for?

Specific antigens (proteins)

What is Immunohistochemistry stains used for?

Bile/bilirubin

What is Hall's bile used for?

Fungi

What is grocotts methenamine silver used for?

Melanin

What is Masson Fontana used for?

Lipids

What is Oil Red O used for?

H&E, WVG, Masson trichrome, Alcian blue, Mucicarmine

What stains can use a section of small intestine as a control?

VVG

What stains can use aorta as a control?

Gordon and Sweets, PAS

What stains can use Liver as a control?

Bacteria

What must be present in Brown and Brenn's control slide?

Bile/bilirubin

What must be present in Hall's Bile control stain?

Antigens

What must be present in IHC control stain?

Fungi

What must be present in GMS control slide?

Melanin/argentaffin granules

What must be present in Masson Fontanas control slide?

Lipids

What must be present in Oil red O control slide?