Lab 4: Enzyme Activity & Micropipetting

These flashcards cover key terms and concepts related to enzyme activity, micropipetting, and indicators based on the lecture notes.

Enzyme Activity

The rate at which an enzyme catalyzes a reaction.

Micropipetting

A technique used to measure and transfer small volumes of liquid.

Hydrolytic enzymes

Enzymes that catalyze the hydrolysis of chemical bonds.

Starch

A polysaccharide composed of glucose monomers.

Maltose

A disaccharide formed from two glucose monomers.

Indicator

A substance that changes color to indicate the presence or absence of another substance.

Lugol’s Solution

An indicator that detects the presence of starch by turning black-purple.

Benedict’s Solution

An indicator that detects the presence of maltose by turning orange-red.

Amylase

An enzyme that catalyzes the hydrolysis of starch into sugars.

Trypsin

An enzyme that catalyzes the hydrolysis of proteins into peptides.

BAPNA

A synthetic substrate for trypsin that produces a yellow dye upon reaction.

Lipase

An enzyme that catalyzes the breakdown of fats into fatty acids and glycerol.

pH Indicator

A substance that shows the acidity or basicity of a solution.

Activation Energy

The minimum energy required for a chemical reaction to occur.

Transition State

An unstable intermediate state during a chemical reaction.

Thermodynamically Spontaneous

A reaction that can occur without the input of additional energy.

Kinetic Energy

The energy of an object due to its motion, affecting molecular collisions.

Enzyme Regulation

The process by which a cell controls the activity or production of enzymes.