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40 Terms
Term
Transformation
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The step in DNA recombinant technique where the uptake of recombinant DNA into a host bacterium is facilitated through heat shock, electroporation, or chemical methods.
Term
Bacillus thuringienesis
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The program used in the production of pest-resistant corn and eggplant in recombinant DNA technology.
Term
Escherichia coli and Saccharomyces cerevisiae
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The organisms used in the production of insulin in recombinant DNA technology.
Term
Designing a primer
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The step in PCR where online bioinformatics tools are helpful in designing a primer.
Term
to separate DNA strands
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The purpose of the denaturation step in PCR.
Term
Provide a starting point for DNA synthesis
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The role of primers in a PCR reaction.
Term
primers bind to the target DNA
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The annealing temperature in PCR is the temperature at which primers bind to the target DNA.
Term
extension
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The final step in a PCR cycle.
Term
RNA-dependent
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The flow of genetic information involved in RT-PCR is RNA-dependent.
Term
Replication
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The component of the flow of genetic information that involves Taq polymerase.
Term
Thermostable
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To be useful in PCR, polymerases from microbes should be thermostable.
Term
exponential
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The phase of DNA amplification that is relevant to the detection of viral pathogens.
Term
Designing a primer
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The most important step in conducting a polymerase chain reaction experiment.
Term
Chemical modification of specific bases
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A key principle underlying Maxam-Gilbert DNA sequencing.
Term
to separate DNA strands
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The purpose of denaturation in Maxam-Gilbert sequencing.
Term
Nucleotide bases are chemically modified then DNA fragments are electrophoresed
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In the Maxam-Gilbert DNA technique, this is how the base sequence is determined.
Term
Polymerase chain reaction terminated by terminator nucleotides then electrophoresed
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In the Sanger technique, the exact sequences of nucleotide bases are determined.
Term
Sequencing by synthesis
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The earliest version of next-generation sequencing.
Term
It does not require a thermal cycler
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NOT true about quantitative PCR (qPCR).
Term
to visualize and confirm amplification
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The purpose of the gel electrophoresis of PCR products.