Phlebotomy order of draw {nha standard}

Yellow tube

specimen whole blood

blood culture bottles aerobic with O2 anaerobic no O2

sodium polyanethol sulfonate (sps)

Light blue tube filled 9:1 ratio

specimen plasma

use for pts on anti coagulants, surgical pt

test;PTT (heparin) PT/INR protime (coumadin warfarin) invert 3 x fibrinogen

sodium citrate

Red tube (glass tube no activator)

specimen serum, test; serology ex HIV, serology tests (RPR, monospot, rheumatoid factor, ANA) blood bank invert 5 x

serum separator tube (sst) Tiger tube

specimen plasma

test; chemistry test glucose, cholesterol, triglycerides, potassium invert 5x

clot activator/polymer gel

Green tube

specimen plasma test; stat chemistry, electroytes, amonia, ABG's, chromosome screening

heparin or sodium heparin

lavender tube specimen;whole blood

test; CBC, RBC, WBC, platelet count, wbc differential count, hemoglobin & hematocrit determinations, ESR(erythrocyte sedimentation rate), sickle cell screening, invert 8-10x HGB, A1C

EDTA(ethylenediaminetraacetic acid)

Pink specimen whole blood or plasma

test; antibody screening (type and screen) compatability test(crossmatch) blood bank

K 2EDTA

Grey tube specimen plasma

test; fasting blood sugar(FBS) glucose tollerance test (GTT) Blood alcohol levels (BAC) lactic acid measurement

potassium oxalate and sodium flouride

Royal blue specimen serum or plasma

test; for trace elements, toxicology, and nutritional chemistry testing

heparin, EDTA or none