microbio lab practical 1 checklist

know the type of shoes and how to wear hair when working in the lab

• close toed shoes

• hair tied back

what two items do you wear when working with bacteria?

• lab coat and gloves

what other item do you need to wear when working with liquids?

• safety glasses

what three items do you store in your bag before entering the lab?

• food

• drinks

• electronic devices

what do you wash your lab bench with at the beginning and end of the class?

• 70% ethanol alcohol

fire extinguisher

• to put out small fires

fire blanket?

• quickly smothers a fire, especially on people and clothing

eyewash station

• if a chemical comes in contact with the eyes

areas to dispose of biological waste

• biohazard can (kill bucket)

• kill rack

• bleach water bath

• glass disposal box

• regular trash can

biohazard can (kill bucket)

• items contaminated with bacteria that are disposable & not made of glass

• petri dish, cotton swab, pipettes

kill rack

• dispose of all bacteria cultures in test tubes (all test tubes)

bleach water bath

• dispose of all microscope slides that have live bacteria like a wet mount

glass disposal box

• dispose any broken glass or heat fixed microscope slides (bacteria are dead)

regular trash can

• any non-glass item without a live culture like gloves or paper towels used to clean lab bench

• remember: if these were used to clean a live culture spill, they go in the biohazard can instead

what device is used to kill the microrganisms found in the biological waste (biohazard can and kill rack)

• ask professor 1

when do you wash your hands?

• before starting the lab

• anytime your hand is in contact with a potential hazard

• most importantly, living organisms that come from any specimens you are working with

• after the lab is completed

• before leaving the lab

how long should you scrub your hands when washing them?

• 20 seconds

when carrying the microscope, on which two parts of the scope will you place your hands?

• one on the arm and one under the base

which objective lens is facing down when starting to focus on a specimen?

• 4x

which adjustment knob is used with the scanning lens?

• course adjustment knob

which adjustment knob is used with the low magnification, high dry, and oil immersion lens?

• fine adjustment knob

which lens is always facing down when storing the scope?

• scanning objective lens

which position the stage should be when storing the scope?

• down

what type of cells are bacteria? are they unicellular or multicellular organisms?

• prokaryotic

• unicellular

for the picture of Spirogyra, be able to name the organism or kingdom, and what the little dot is in the picture

• organism: Spirogyra

• kingdom: protista

• little dot: nucleus

for the Amoeba model, be able to state its kingdom and label the following structures:

• kingdom: protista

• cell membrane, nucleus, food vacuole, DNA and pseudopod

for the five eukaryotes below, when looking at the picture, be able to write the name of the organism with proper capitalization and spelling. also be able to name the kingdom an if it is unicellular or multicellular

• Acanthamoeba

• Candida

• Aspergillus

• Trichinella spiralis

• Taenia saginata

For the picture of Acanthamoeba, also know the structure it uses for movement (be able to identify it)

• pseudopod

For the picture of Candida, also know the body form

• yeast

For the picture of Aspergillus, also know the body form, and identify the mycelium and conidia

body form: mycelium

for the picture of Trichinella spiralis, also identify the larva

• point A is larva

for the two Taenia saginata pictures be able to identify the region as either the scolex or proglottid

• scolex is the end or beginning

• proglottid is the body

know viruses are acellular***. what is the cell called that it infects (enters)?

• a host cell

for the viral model, be able to identify the bacterial cell, viral capsid, and viral genome

• bacterial cell:

  • clear rectangular structure

  • host cell

• viral capsid:

  • clear structure

  • made of protein

• viral genome

  • brown rod

  • DNA

for each model, identify the correct stage of the lytic cycle

• adsorption → penetration → viral DNA replication → protein production or synthesis → assembly → release

know why aseptic technique is used

• to prevent contamination

which specimen preparation allows for viewing live cells and will tell if a microorganism is motile?

• wet mount

what structure does motile bacteria have?

• flagella

what is the type of movement in motile bacteria?

• straight run & tumble

what type of movement is found in non-motile bacteria?

• Brownian motive

after making a smear prep, are living or dead cells viewed?

• dead

know the charge of bacterial cells. does positive dye stain the bacteria or stain the background? what about negative dye?

• positive = bacteria

• negative = background

what tool is used to transfer bacteria when making a smear prep?

• inoculation loop

when using aseptic technique when making a smear prep, which two items are flamed?

• open test tubes and inoculating loops

what are the two purposes for heat fixing the slide?

• to kill the bacteria and adhere the bacteria to the slide

what was the name of the dye used for the simple stain?

• methylene blue