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Features of mycobacterium tuberculosis:
Produces niacin
Produces heat sensitive catalase
Reservoir of M.TB?
Human lungs
What stain is used specifically for detection of Mycobacterium?
Auramine-rhodamine stain
Tuberculosis prevention?
Immunoprophylaxis: administration of the attenuated strain derived from M.bovis strain
Isoniazid: taken for 9 months, can prevent TB in person with infection but no clinical symptoms
Features of corynebacterium diphtheriae?
Gram positive club shaped rods
V and L arrangement (Chinese characters)
Volutin granules on Loeffler medium
Aerobic bacteria
Disease caused by corynebacterium diphtheriae?
Throat diphtheriae with dirty grey pseudomembrane
Complications associated with disease caused by corynebacterium diphtheriae?
Myocarditis
Neuritis - paralysis
Kidney problems
Bleeding problems
Diagnosis of Actinomycosis?
Gram staining of the granules
Anaerobic culture, selective media: with NaCl, horse blood, MTZ, nalidixic acid
Colonial morphology - ‘molar tooth’
‘Sun ray’ appearance occurs on crushing bacilli which release the sulphur granules between slides