Eukaryotic Genes and Genomes III

Vocabulary flashcards based on key terms and concepts from the lecture on Eukaryotic Genes and Genomes.

Cis-acting sequences

Regulatory elements that are located on the same molecule of DNA as the gene they regulate.

Glucose repression

A phenomenon where the presence of glucose inhibits the expression of genes, such as the Gal1 gene.

Gal1 gene

A gene in yeast that codes for galactokinase and is regulated by glucose and galactose.

UAS (Upstream Activation Sequence)

A cis-acting element that helps to activate the transcription of a gene when bound by a transcription factor.

TATA sequence

A conserved DNA sequence required for the assembly of the basal transcription machinery.

Deletion analysis

A technique used to identify important sequences within a promoter by systematically removing sections of DNA.

Mig1 protein

A transcription factor that binds to URSGAL and is involved in glucose repression of gene expression.

Snf1 complex

A kinase that regulates the activity of the Mig1 repressor by phosphorylating it based on glucose levels.

URSGAL (Upstream Repressor Sequence)

A sequence element required for repression by glucose, specific to galactose regulation.

Yeast two-hybrid assay

A method used to study protein-protein interactions in yeast by fusing proteins to Gal4 activation and binding domains.