Polymerase Chain Reaction

A technique used to rapidly isolate & amplify a specific sequence of DNA

PCR ( Polymerase Chain Reaction)

Unlike DNA cloning which uses living cells, PCR takes place in a : ________

Test tube

Why do we use PCR?

Its a quick & efficient process for generating many identical copies of DNA in a test tube

Components of PCR mixture include:

• Template DNA

• DNA Primers

• Thermostable DNA Polymerase

• 4 Nucleotides

The polymerase the template sequence of DNA with each cycle:

Doubles the template sequence of DNA with each cycle

Lists the steps in order for one cycle of the PCR?

• Denature DNA

• Anneal primers

• Extend primer’s utilizing a thermostable DNA polymerase

Why is a DNA polymerase from a thermophilic bacterium used in PCR?

Thermophilic DNA polymerase can withstand high temperatures that denature most proteins

Why do the fragments of DNA in gel electrophoresis move away from the negative cathode ?

DNA is negatively charged and attracted to positive anode