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Binomial Nomenclature
The system of giving every organism two names: the Genus (capitalized) and the specific epithet (species name, not capitalized)
Staphylococcus aureus (Name Meaning)
“Staphylo” describes clustered arrangement
Bergey’s Manual
The reference used for bacterial classification
Strain
A collection of cells derived from a single cell within a species
Phylogenetic Hierarchy (Order)
Species → Genus → Family → Order → Class → Division/Phylum → Kingdom → Domain
Procaryotes
Simple cells (bacteria and cyanobacteria) that exhibit all characteristics of life but lack a complex system of membranes and membrane-bound organelles.
Cocci Arrangements
Cocci that remain attached form: diplococci (pairs), streptococci (chains), tetrads (groups of 4), sarcinae (groups of 8), or staphylococci (clusters).
Bacilli Arrangements
Rod-shaped bacteria that appear in pairs are diplobacilli
Spiral Bacteria
Curved rods are vibrios
Monomorphic vs. Pleomorphic
Monomorphic bacteria always maintain the same shape
Peptidoglycan
A net-like polymer (sacculus) forming the bacterial cell wall, made of repeating N-acetylglucosamine (GlcNAc) and N-acetylmuramic acid (MurNAc) units linked by β-1,4 glycosidic bonds and cross-linked by short peptide chains.
Gram-Positive Cell Wall
Relatively thick (30–100 nm)
Gram-Negative Cell Wall
Thinner and more chemically complex than Gram-positive
Lipopolysaccharide (LPS)
A component of the outer membrane of Gram-negative bacteria
Plasma Membrane
Composed of phospholipids (with a polar hydrophilic phosphate head and hydrophobic fatty acid tails) and proteins
Fimbriae (Pili)
Hair-like proteinaceous structures (2–3 nm diameter, 0.1 µm to several µm long) projecting from Gram-negative cell surfaces
Flagella
Made of proteins called flagellins
Capsule (Slime Layer)
Composed of polysaccharide surrounding the bacterial cell
Plasmid
A short, usually circular, double-stranded DNA segment found in the cytoplasm separate from the main chromosome
Nucleoid
A region of the cytoplasm (not membrane-bound) where the bacterial chromosome (usually a single circular DNA strand) is located
Ribosomes (Bacteria)
Bacteria contain approximately 20,000 ribosomes per cell
Metabolism
The sum total of all chemical reactions taking place in a living cell
Catabolism
Energy-releasing reactions involving the breakdown of complex organic compounds into simpler ones (e.g., breakdown of sugars into CO₂ and H₂O)
Anabolism
Energy-requiring (biosynthetic) reactions that build complex organic molecules from simpler ones (e.g., proteins from amino acids, nucleic acids from nucleotides, polysaccharides from simple sugars).
ATP (Adenosine Triphosphate)
The energy currency of the cell
ATP–ADP Cycle
During catabolism, ATP loses a phosphate group to form ADP + energy
Activation Energy
The energy required to initiate a chemical reaction by enabling atoms, ions, or molecules to collide with sufficient impact
Enzymes
Biological catalysts that facilitate cellular chemical reactions without being consumed
Coenzymes (NAD⁺/NADH)
Small molecules that assist enzymes in transferring electrons and protons in redox reactions
Focal Metabolites
A few key precursor molecules from which biosynthetic building blocks and coenzymes can be traced