Microbiology and Parasitology Lecture Review

Comprehensive vocabulary flashcards covering bacterial identification, odors, motility, culture media, and clinical microbiology based on the Sushi Notes 2.0 transcript.

Alcaligenes faecalis Odor

Fruity apple/strawberries

Proteus spp. Odor

Burnt chocolate odor/ Gunpowder

Pseudomonas aeruginosa Odor

Fruity (grape-like)/ Corn-tortilla

Pasteurella multocida Odor

Mushroom/musty odor

Stenotrophomonas maltophilia Odor

Mousy or ammonia-like

Eikenella corrodens Odor

Bleach-like

Chromobacterium violaceum Odor

Almond, ammonium cyanide

Streptococcus anginosus Odor

Butterscotch/sweet honeysuckle

Pseudomonas aeruginosa Motility

Triple motility: Swimming, Swarming, Twitching

Campylobacter Motility

Darting

Vibrio Motility

Rapid darting/shooting star

Listeria Motility

Tumbling

Spirochetes Motility

Corkscrew

Gram Stain Secondary Stain for Legionella

$0.1\%$ fuchsin

Acid-fast Primary Stain

Carbolfuchsin

Bile Esculin Agar (BEA)

Differential and presumptive ID of group D Streptococci and enterococci

Buffered Charcoal Yeast Extract agar (BCYE)

Enrichment and selection for Legionella spp.

Cefsulodin-irgasan-novobiocin (CIN) agar

Selective for Yersinia spp.; may be useful for isolation of Aeromonas (Bull's eye colonies)

Thiosulfate citrate-bile salt (TCBS) agar

Selective and differential for Vibrio spp.

MacConkey sorbitol agar (SMAC)

For the selection and differentiation of E. coli $O157:H7$ in stool specimens

Alpha Hemolysis

Incomplete or partial hemolysis; green or brown color surrounding the colony

Beta Hemolysis

Complete hemolysis of red blood cells; clearing or colorless zone around colony

Mycobacterium tuberculosis Colony Appearance

Cauliflower

Actinomyces israelii Colony Appearance

Molar-tooth

Moraxella catarrhalis Colony Appearance

Hockey puck

Bacillus anthracis Colony Appearance

String of pearls (media with penicillin), bamboo pole, and MEDUSA HEAD

Bordetella pertussis Colony Appearance

Mercury drop, pearls

Schick test

Diphtheria skin test

Elek's test

Toxigenic test for toxigenic C. diphtheriae

Dick's test

Scarlet fever skin test, positive result is red

Hansen's disease

Associated with Mycobacterium leprae

Waterhouse-Friedrichsen syndrome

Associated with Neisseria meningitidis

Woolsorter's disease

Associated with Bacillus anthracis

Staphylococcus aureus Clinical Signficance

1 cause of FOOD POISONING (symptoms= $2-6$ hrs), skin infection, and osteomyelitis

Streptococcus agalactiae Clinical Significance

Major pathogen of newborn, #1 cause of neonatal meningitis

UTI Diagnostic Threshold

$$> 100,000\,CFU/mL$$

Pfeiffer's bacillus

Common name for Haemophilus influenzae (XYLOSE POSITIVE)

Koch-Weeks bacillus

Common name for Haemophilus aegypticus

Bang's bacillus

Common name for Brucella abortus

Photochromogens (Group I)

Mycobacteria that produce yellow pigment when exposed to light; takes $> 7$ days to appear (e.g., M. kansasii, M. asiaticum, M. marinum)

Autoclave Quality Control

SPORE test weekly using Bacillus stearothermophilus; must reach $121^{\circ}C$ with $15\,psi$

0.5 McFarland standard

Equivalent to $1.5 \times 10^8\,CFU/mL$; composed of $1\%$ Sulfuric acid and $1.175\%$ Barium chloride

mecA gene

Gene used for the molecular detection of MRSA

D test

Detects inducible clindamycin resistance; a D shaped zone of inhibition means erythromycin induced clindamycin resistance

Malassezia furfur

Causes Tinea versicolor; lipophilic, apple-green fluorescence under Wood's lamp, 'spaghetti and meatball' microscopic appearance

Sporothrix schenckii Morphology

Yeast: Cigar-shape; Mold: Flowerette

H2S Positive Organisms (SPACE)

Salmonella, Proteus, Arizona, Citrobacter, Edwardsiella