key proteins and markers

Synaptophysin

A synaptic vesicle protein that serves as a marker for presynaptic terminals and is involved in neurotransmitter release.

Neurotrophin-3 (NT-3)

A protein that supports the growth and differentiation of neurons, particularly during development.

Caspase-3

An enzyme that plays a crucial role in the execution phase of cell apoptosis, often studied in neurodegenerative diseases.

GAPDH

loading control

MAP2

dendritic/soma protein - common marker in staining

MAPT

microtubule associated protein tau, axonal marker

G3BP1

core stress granule protein/marker

LC3 1

autophagy marker

LC3 2

autophagy marker 2

tomm20

mitochondria marker

IL - 6

pro-inflammatory cytokine

PINK1

mitochondria-associated kinase; degraded when mitochondria are healthy

Parkin

a protein that plays a role in mitophagy

cAMP

intracellular second messenger

M6P

signal for transport to lysosomal membrane