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(2) Pyruvate to Phospoenolpyruvate: Energetically expensive
1st step: Pyruvate to oxaloacetate using pyruvate carboxylase (carboxylation) using 2 ATP (1 per pyruvate molecule)
2nd step: Oxaloacetate to phosphoenolpyruvate using phosphoenolpyruvate carboxykinase (decarboxylation) using 2 GTP (1 per oxcaloacetate molecule)
Fructose 1,6 - biphosphate to Fructose 6 phospate
Fructose 1,6 - biphosphatase-1 removes phosphate group at carbon 1 (no ATP produced)
Glucose 6-phosphate to Glucose
Glucose 6-phosphatase removes a phosphate group at carbon 6
How many H20 molecules used?
6 (trust me bro)
How many Pi produced?
6 (trust me bro)
Why is there no CO2 in net reaction?
Used up and produced cancel each other out
Glycolysis Net Reaction and free energy
Glucose + 2ADP + 2NAD+ + 2Pi —→ 2Pyruvate + 2ATP + 2NADH + 2H+ + 2H2O; -84 kJ/mol
Gluconeogenesis Net Reaction and Free energy
2Pyruvate + 4ATP + 2GTP + 2NADH + 2H+ + 6H2O —→ Glucose + 4ADP + 2GDP + 2NAD+ + 6 Pi ; -38 kJ/mol