Mass Spectrometry Module Revision Questions

These flashcards cover key concepts, definitions, and analytical techniques related to mass spectrometry as outlined in the lecture notes.

What are the three main techniques to choose from for analyzing trace metals in high solid samples?

ICP-MS, GC-MS, and LC-MS.

Which mass analyzer would be best suited for elemental composition analysis by accurate mass?

TOF or Orbitrap.

What is the equation for calculating Mass Resolution, and what does Δm represent?

Mass Resolution (R) = m/Δm, where Δm represents the width of the mass peak.

How do you calculate ppm error in Mass Accuracy?

ppm error is calculated using the formula: (Measured mass - Theoretical mass) / Theoretical mass × 10^6.

What are identification points in analytical method development?

They are parameters or criteria used to confirm the identity of an analyte, important for ensuring reliable results.

What is the purpose of using quantification and qualification ions in mass spectrometry?

Quantification ions are used for measuring concentration, while qualification ions help confirm the presence of the analyte.

What advantages does LC-MS have for analyzing hydrophilic non-volatile compounds?

It effectively separates compounds before mass analysis, which is beneficial for polar and non-volatile substances.

What type of mass analyzer is commonly used for ICP-MS?

Both Low Resolution (LR) and High Resolution (HR) mass analyzers are commonly utilized.

What are the two methods of GC-MS in terms of data acquisition?

Full scan (Total Ion Chromatogram) and Selected Ion Monitoring (SIM).

Why is mass spectrometry MS/MS more selective than full scan for complex matrices?

MS/MS allows for targeted fragmentation of analytes, improving specificity and reducing background noise.