MOLBIO LAB 1

Handwash duration

40-60 seconds

Handrub duration

20-30 seconds

Eating inside the laboratory is prohibited because it may lead to what pathogen exposure route associated with ingestion of contaminated materials?

Fecal-Oral Exposure to Pathogens

What laboratory safety practice protects personnel from chemicals and biohazards and prevents contamination of patient samples?

Personal Protective Equipment (PPE)

What biosafety level prevents exposure through percutaneous injury and mucous membranes when handling biological hazards?

Biosafety Level 2

What type of protective equipment should be worn when working with ultraviolet light to prevent eye injury and facial exposure?

Eye and Face Shields

Short exposure to ultraviolet light in the laboratory may result in what two types of injuries?

Severe Burns and Eye Damage

Chemical Hazards

Acrylamide; Chloroform; Ethidium bromide; Formamide; Phenol

Electrical hazard associated with the electrophoresis power supply

Increased Shock Hazard

Physical Methods of Sterilization

Incineration; Moist heat; Dry heat; Filtration; Ionizing gamma radiation

Most common physical method of sterilization

Incineration

Fastest and simplest sterilization method using steam under pressure

Moist heat

Principle of Moist heat

Steam under pressure

Physical Methods of Disinfection

Boiling; Pasteurization; Ultra violet light

Black

Non-infectious dry waste

Green

Non-infectious wet waste

Yellow

Infectious and Pathological waste

Yellow with black band

Chemical waste including heavy metals

Orange

Radioactive waste

Red

Sharps and pressurized containers