Micro bio 215- Practical 2

Pos. Presumptive ferment test

water sample might contain fecal coliforms

1/10=

0.1 = 10-^1

  1/100=

0.01= 10-^2  

1/1000=   

0.001=10-^3

1/10000=

0.0001=10-^4

MPN

Most Probable Number

The definition of Fecal Coliforms is: 

Gram - neg, Rob- shape, Ferment lactose, Produce acid, No endospores

Fecal coliform has turned pink on this agar. What is the name of this agar?

MacConkey

PATHOGENIC BACTERIA:

Bacillus anthracis, respiratory disease

PATHOGENIC BACTERIA:

Neisseria gonorrhoeae, disease gonorrhea

PATHOGENIC BACTERIA:

Treponema pallidum, disease syphilis

PATHOGENIC BACTERIA:

Corynebacterium diphtheriae, disease diphtheria

PATHOGENIC BACTERIA:

Clostridium, Cdiff

PATHOGENIC BACTERIA:

Staphylococcus aureus, disease MRSA

PATHOGENIC BACTERIA:

Streptococcus pneumoniae, disease pneumonia

PATHOGENIC PROTISTS: (diarrhea w/ blood)

Entamoeba histolytica, disease amoebic dysentery

PATHOGENIC PROTISTS:

Balantidium coli, disease balantidial dysentery

PATHOGENIC PROTISTS:

Trypanosoma, disease sleeping sickness

PATHOGENIC PROTISTS:

Plasmodium, disease malaria

Pasteurization

do not kill ALL bacteria (classic, HTST, UHT)

Pasteurization- CLASSIC

High temp, short time; 145F 30mins

Pasteurization- HTST

161F 15secs

Pasteurization- UHT (ultra high temp)

284F 3secs

Autoclave

250F 15mins Kills ALL bacteria (sterile)

autoclave tape

non-sterilized (top) and sterilized (bottom).

Endospore stain of Bacillus

endospores-GREEN dots and vegetative cells-PINK/RED cells.

For Endospore Stain what was used?

Malachite green

For vegetative cells what was used?

safranin

__

Bacillus