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What is the first rule
You should always carry a microscope with two hands one hand on the arm and the other hand under the base.
What is the second rule
You should always start focusing a microscope on the lowest power and leave the microscope on the low power lens when you finish using it.
What is the rule regarding how to leave the microscope when you are finished?
You should always leave the microscope on the low power lens when you finish using it.
What is the third rule
You should use the coarse adjustment only to focus the microscope at first on low power, then use the fine to get the image into perfect focus.
What is the rule regarding the use of the fine adjustment knob?
Use it to get the image into perfect focus.
What is the specific rule for focusing while using the high power lens?
Always use the fine adjustment
What is the 4th rule
Always put the dust cover back on the microscope when you are not using it.
Why is it called a compound light microscope?
It uses two lenses the ocular lens and the objective lens for greater magnification.
What is the definition of magnification?
The degree to which an object is made to appear larger without altering its actual size.
What is the definition of resolution?
The ability of a lens system to show fine detail and distinguish between two points.
How do you adjust the resolution on a microscope?
Maximize the light and use the fine adjustment knob.
What is the field of view?
The amount of area you see through the lens that decreases and magnification increases.
What is the function of the Ocular Lens Eyepiece?
The lens at the top that you look through that is magnified
What is the function of the Body Tube?
It holds the eyepiece and allows light to pass from the objective to the eyepiece.
What is the function of the Revolving Nosepiece?
It holds the objective lenses in place
What is the function of the Objective Lenses?
to magnify the specimen.
What is the function of the Stage?
The flat area where you place your slide or specimen.
What is the function of the Stage Clips?
They hold the microscope slide in place on the stage.
What is the function of the Diaphragm?
It regulates the amount of light that passes through the stage and specimen.
What is the function of the Coarse Adjustment Knob?
It moves the stage up and down to provide initial clarity and moves the focus faster.
What is the function of the Light Source Illuminator?
It provides the light needed to see the specimen.
How do you determine the total magnification of a microscope?
Multiply the eyepiece power usually 10x by the power of the objective lens.
How should you lower the coverslip when making a wet mount to avoid air bubbles?
hold the coverslip at a 45-degree angle to the slide, touching one edge of the liquid sample first, and then slowly lower it
In what two ways does the image through the microscope differ from the actual specimen?
The image is upside down and flipped reversed inverted.
What happens to the amount of light when switching from low to high power?
the light intensity significantly decreases, making the image appear smaller
Why should you keep both eyes open while looking into the microscope?
This helps you to avoid a painful condition called eyestrain.
Why must a specimen be centered on low power before moving to high power?
Because the microscope zooms in so much that the object becomes unclear or lost if not centered.