Laboratory Simulation on PCR Procedures

A collection of vocabulary flashcards based on laboratory simulation notes for PCR procedures.

Micropipette

A laboratory tool used to measure and transfer small volumes of liquid.

Master Mix

A premade solution containing all necessary components for PCR, excluding the DNA template.

PCR Cycle

A series of temperature changes used to amplify DNA in PCR.

Loading Dye

A colored solution added to samples before gel electrophoresis to track the progress and ensure proper loading.

Electrophoresis

A technique used to separate DNA fragments based on their size by applying an electric field.

Amplication

The process of increasing the quantity of a specific DNA sequence during PCR.

Taq Polymerase

A heat-stable enzyme used in PCR to synthesize DNA strands.

dNTP Mix

A mixture of deoxynucleotide triphosphates used as building blocks for DNA synthesis.

10x Taq Buffer

A concentrated solution that provides the necessary ionic environment for the Taq Polymerase during PCR.

Deionized Water

Purified water used to dilute reagents or prepare solutions in PCR.

Primer

Short sequences of nucleotides that provide a starting point for DNA synthesis in PCR.

Visualization

The process of using imaging techniques to observe DNA bands in gel electrophoresis.

Neisseria meningitidis

A bacterium often detected in PCR tests to identify infections.

Sample

A small quantity of material collected for analysis or testing in a laboratory.

Heat Stable

Refers to substances, like Taq Polymerase, that remain functional at high temperatures.

Master Mix Solution Components

Includes Taq polymerase, primers, dNTPs, buffer, and deionized water.

PCR Result Interpretation

Analyzing outcomes of PCR to determine if the target DNA was amplified successfully.

Electrophoresis Result Null

Occurs when no DNA bands are detected after running gel electrophoresis.

Phase

A distinct stage in a laboratory simulation or experimentation process.

Incorrect Attempts

Refers to how many times a question was answered incorrectly in the simulation.

Gel Loading

The process of placing DNA samples into a gel for electrophoresis study.