Bio Lab Past Quiz Questions

BRCA1 and BRCA2 genes normally act as tumor suppressors, by helping in  regulation of cell division

• True

• False

True

The picture of chromosome below shows the P arm of the chromosome. 

• True

• False

True

The following sequence is considered to be in FASTA format.

>NP_009225.1 breast cancer type 1 susceptibility protein isoform 1 [Homo sapiens]

MDLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVSIIQSMGYRNRAKRLLQS

• True

• False

True

Which of the following databases is publically available to find variations in a gene that has clinical significance

• Nucleotide

• Protein

• ClinVar

• Domains and structures

ClinVar

In bioinformatics, Query coverage refers to the value given as a percentage (0-100%), how much of the length of your sequence of interest (query sequence) aligns with the length of a reference sequence

• True

• False

True

The direction of adding nucleotides in a PCR reaction is from 3' to 5' end of the template DNA

• True

• False

False

The purpose of adding bromophenol blue to the DNA loading dye is to

• Allow monitoring the DNA migration on the gel during electrophoresis

• Give density to the DNA so it can sink in the wells

• Change negative charge of DNA to a net positive charge

• Resist pH changes for running the gel

Allow monitoring the DNA migration on the gel during electrophoresis

As you learned in the fast plant genotyping lab, the wild type should give a band for 280 bp and if there is mutation, it should give a band for 150bp. Based on the figure below what is the genotype of the seedling in lane # 4 with the arrow that has two bands

• Wild type

• Homozygous for DFR (green)

• Heterozygous for wild type and DFR

Heterozygous for wild type and DFR

The insertion in the DFR gene leads to green colored seedling

• True

• False

True

While screening plants for segregation of a phenotype/trait this generation of seeds/plants is used

• T1

• T2

• T3

• A cross between T1 and parents (back cross)

T2

Which one the following is NOT a characteristic of VNTRs?

• Useful in genetics, biology research, forensics and DNA fingerprinting

• Repeats vary in sizes from as low as 6 bp to as much as100 bp 

• They cut DNA at specific sites

• These loci contain DNA sequences organized as a tandem repeats

They cut DNA at specific sites

In a population that is not evolving, the frequencies of different alleles within a population are expected to remain the same over time

• True

• False

True

You are studying a population in Hardy-Weinberg equilibrium, for a gene with two alleles, p and q.  The frequency of p allele is 0.46 then what would be the frequency of the heterozygous

• 0.2116

• 0.54

• 0.29

• 0.496

0.496

The gene you are studying in the lab for sleep experiment is 

• GPCR

• PER 3

• TAS2R38

• CRY

PER 3

Per3 carrying 4 copies of this repeat may be associated with a preference for evening, while having 5 repeats may be associated with a preference for morning

• True

• False

True

The function of isopropanol in the miniprep DNA isolation we performed in the lab is 

• Remove excess salts 

• Solubilize membranes

• Precipitate DNA 

• Alkaline lysis

Precipitate DNA 

If you transformed bacteria with a plasmid carrying the kanamycin (Kan) resistance gene and a gene for Lac Z under the control of an arabinose-dependent promoter, what media should you use to observe the blue colored colonies?

• LB+ Kan

• LB+Kan+X-gal

• LB+Kan+Arabinose

• LB+Kan+X-gal+Arabinose

LB+Kan+X-gal+Arabinose

In the bacterial transformation lab, you transformed E. coli with the pRK242 plasmid. You were very happy to see colonies the next day on your plate. Now you isolated plasmid DNA from the cultures of your transformed cells and want to make sure the plasmid is correct. You set up a restriction digest with an enzyme BamHI to validate the plasmid. Here is the plasmid map, based on this what sizes of fragments do you expect to see on your gel?

• 400bp, 1500bp and a 3800 bp

• 2700 bp and 2300bp

• 100bp and 2800bp

• 2700 bp and 2200bp

2700 bp and 2300bp

You and your friend isolated plasmid DNA from a bactureail culture and measured the concentration on a nanodrop. The concentration your of the plasmid is 500ng/ul with a 260/280 ration of 2.02 and that of your friend's is 15ng/ul. Since your DNA has better concentration you offer your your plasmid DNA to your friend. You both use 1 ug of this DNA to perform two restriction enzyme digests that should give you two bands. Your friend ran their gel and saw two nice bands, but your gel looks like the one below.

What might have gone wrong with the gel?

• Forgot to add SYBR Safe 

• Ran the gel too long

• Placed the gel towards the wrong electrodes

• My sample did not have enough DNA

Placed the gel towards the wrong electrodes

The restriction enzyme EcoRI cuts the DNA at specific sites which have palindromic DNA sequences

• True

• False

True

In E. coli Lac Z gene codes for Cas-9 endonuclease

• True

• False

False

In a CRISPR mediated gene editing experiment, which one the components is required for  recognizing the gene to be edited

• Lac Z

• Cas-9

• sgRNA

• Donor DNA

sgRNA

Which of the following reactions will have an edited gene

• The reaction that has donor DNA and guide RNA

• The reaction that has donor DNA,  guide RNA, HDR machinery

• The reaction that has donor DNA,  guide RNA, HDR machinery and Cas-9 nuclease

• The reaction that has Cas -9, donor DNA and guide RNA 

The reaction that has donor DNA,  guide RNA, HDR machinery and Cas-9 nuclease

Protospacer is the DNA region that is targeted for cleavage by the CRISPR system

• True

• False

True

In prokaryotes, the CRISPR-Cas9 microbial defense system this sequence is inserted in CRISPR region of the bacterial genome as a signature for future infections

• Spacer

• Cas-9

• Donor'

• HDR

Spacer