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Cell membrane
Selectivley permeable barrier that regulates movement in and out the cell

What is the cell membrane made of
A phospholipid bilayer
membrane proteins,
carbs
and cholesterol
Bi pro car chol

What does the cell membrane regulate
Cell signaling and communication

What do the hydtophobic tails of the phospholipid bilayer act as
A barrier for water soluble substances

What are Membrane proteins used for
Facilitate transport, used as receptors, and provide structural support
Transport reception support

Integral proteins:
Embedded in the membrane
amphipathic proteins that aid in
signaling and transportation

Transmembrane protiens
integral proteins that span the entire membrane

peripheral membrane proteins
On the membrane surface,
hydrophillic

What are periperal membrane proteins used for
receptors, cell recognition, enzymes, or adhesion

Where are carbs attached on the cell membrane
the surface

What are carbs used for on the cell surface
Recogniation, signaling, adhesion

Where is cholesterol found?
In cell membrane, but only eukaryotic
agonist
molecules that bind to receptors to activate a biological response

antagonist
molecules that bind to receptors to inhibita biological response

how do proteins promote adhesion
they attach cells to other things and anchor the cytoskeleton

how do proteins promote cellular recognition
glycoproteins

Simple diffusion
small uncharged molecules or lipid soluble molecules
across a concentration gradient (no energy)

Faccilitated tranport
large or charged molecules across concentration gradient using channel proteins
no energy investement

Active transport
molecules across the cell membrane against concentration gradient
need energy investment

Types of active transport
primary and secondary

Primary transport
membrane protiens pump molecules against concentration gradient via ATP hydrolysis

Example of Primary active transport
sodium potassium pump

secondary transport
uses the energy from one molecule moving down its electrochemical gradient to drive transport of another molecule
it requires energy to establish the initial concentration gradient (needs primary active transport )

Endocytosis
Cell membrane internalizes extracellular substances
can be pinocytosis, phagocytosis, or receptor mediated endocytosis


Phagocytosis
Cellular eating

Pinocytosis
Cellular drinking

Receptor-mediated endocytosis
Endocytosis that is initiated by dissolved molecules binding to peripheral membrane receptor proteins
Ex. clathrin mediated endocytosis

Exocytosis
internal substance are released to the extracellular space, internal vesicle fuses to the cell membrane and releases contents

Cytosol
aqueous intracellular fluid

where are organelles found
only in eukaryotes

cytoplasm
cytosol + organelles

What does the nucleus do
protects and stores DNA
houses replication and transcription

Whats the prokaryote equivalent of a nucleus
a nucleoid

Nucleoplasm
Cytoplasm of the nucleus

Nuclear envelope
Encapsulates the nucleus; contains both an inner and outer phospholipid bilayer

Nuclear pores
Channels in the nuclear envelope

Nucleolus
Produces components of ribosomes (rRNA)

How do ribosomes help with protein production
Via translation

What makes up a eukaryotic ribosome
60S and 40S form to create 80S

How are eukaryotic ribosmes created
Nucleolus Creates 60S and 40S subunits,
60S and 40S subunits assemble in the nucleoplasm
They are then taken into the cytosol where they form the full ribosome

What makes up a prokaryotic ribosome
50S and 30S to form (70S)

what do Free floating ribosomes do
produce protiens that function in the cell

what do Rough ER ribosmes do
produce prtoeins for extracellular or cellular membrane use

Endomembrane system
nucleus, smooth and rough ERs, lysosomes, vacuoles, cell membrane
nERm LV

Endomembrane system purpose
Modify, package, and transport
proteins and lipids

Rough ER
organelle attached to the nuclear envelope that has ribosomes on the outside (making it rough)
What do the embedded ribosomes in the rough ER do?
produce proteins to travel to the lumen of the rough ER for modifications

What is the rough ER composed of
Cisternae (flattened sacs)

Where do the proteins prosseced by the rough ER go?
into the cellular membrane or the extracellular space

Smooth ER
Extension of rough ER
sythesizes lipids, steroid hormones, and detoxifies cells

Golgi apparatus purpose
store, export, and modify proteins to be secreted by the cell
STEM

What is the golgi apparatus made of
cisternae

how do proteins enter and exit the golgi apparatus
Enter at the cis face and exit from the trans face

Peroxisomes
perform hydrolysis
break down stored fatty acids, and help with detoxification

What is a side product of perxisomes
hydrogen peroxide, which is broken by catalase

Lysosomes
in animal cells,
break down cellular waste via hydrolytic enzymes, and cause apoptosis

Vacuoles
for storage,
plants use them for rigity or waste breakdown
some protists use it for osmoregulation

How is mitochonrial DNA inherited
maternally

Where does cellular respiration take place?
The mitochondria

Chloroplasts
Type of plastid (doube membranes organelles found in plant cells and algae)
that performs photosynthesis

Where are centrosomes found
animal cells

Centrosomes
Centrioles that act as microtubule organizing centers during cell replication

cytoskeleton
provides structural support within cytoplasm

What makes up the cytoskeleton
Microfilaments
intermediate filaments
and microtubules

Microfilaments
Smallest cytoskeletal structure, made up of double helix of actin

Microfilaments Composition
Made of actin (a contractile protein)

Microfilaments use
cell movement
Cytoplasmic streaming (movement of cytoplasm inside the cell)
Cleavage furrow formation during cell division
Muscle contraction

Cytoplasmic streaming
(movement of cytoplasm inside the cell)

Intermediate Filaments
Medium-sized cytoskeletal filaments used for structural support

Intermediate Filaments composition
Made of a variety of proteins, including keratin

Keratin
found in skin, hair, and nails
in intermediate filaments

Microtubules
Largest cytoskeletal filaments that stabilize and shape the cell

Microtubules Composition
Made of tubulin protein dimers

Microtubules Function
Form centrioles
Essential for cell movement via cilia and flagella

Flagella
long, hair like structures than are used for cell movement, made of microtubules

Cilia
Small, Hair like projections made of Tubulin
in eukaryotes, for movements

Kinesin and dyenin
Proteins that travel on microtubules and are used for transport

Microtubule Organizing Centers
Organize the extension of microtubules in the cell

what are the Key Components of Microtubule Organizing Centers
centrioles, which two make up Centrosomes

Centrosomes
A pair of centrioles oriented at 90-degree angles to each other
that Serve as a hub for microtubule organization

Centrioles
Hollow cylinders made of microtubules
Found in animal cells
2 make up centrosomes

extracellular matrix
Supports the outside of the cell

What is the ECM made of
Protiens and glycoprotiens

What are the components of the ECM
Collagen
proteoglycans
integrins
fibronectin and laminin

Proteoglycans
ECM glycoprotein with a lot of carbs

Collagen
ECM structural protein secreted by fibroblasts

What secretes collagen
Fibroblasts

What polymers do collagen fibers form
collagen fibrils

Integrins
transmembrane proteins that control ECM adhesion and work as cell receptors

Fibronectin
attached to integrins
helps with signal transduction

laminin
attached to integrins
helps with cell differentiation, adhesion, and movement

What do plants have cell walls made of
cellulose

What do fungi have cell walls made of
chitin

What do bacteria have cell walls made of
peptidoglycan

What do archea have cell walls made of
polysaccharides

Cell wall
carb based structures that act like an ECM but more rigid
provide support to cells without ECM or with minimal ECM

Cell matrix juntions
Connect the extracellular matrix to the cytoskeleton
Focal adhesions or hemidesmosomes

Focal adhesions
type of cell matrix junction that uses actin microfilaments

Hemidesmosomes
type of cell matrix junction that uses intermediate filaments
