Math Set 2

1 liter = ________ µl (microliter)

1,000,000

0.5 kg = ________ mg

500,000

20 µl = ________ ml

0.02

57 nmole = __________ µmole

0.057

100 µl of 10mM dNTP solution = ______ nmoles of dNTP

1,000

Grams of NaOH required for 3L of 0.1M solution? (MW = 40 g/mol)

12g

Grams of HEPES required for 500ml of 10mM solution? (MW = 238.3)

1.19g

Grams of LiAc required for 200ml of 100mM solution? (MW = 66)

1.32g

Grams of CaCl2 required for 200ml of 35.4mM solution? (MW = 110.98)

0.786g

Tris Base powder (mg) needed for 400ml of 15mM buffer? (MW = 121.14)

726.84mg

Grams of hydroxyurea needed for 5ml of 2M solution? (MW = 76.06)

0.7606g

To make 400ml of 15mM Tris-HCl from a 1M stock, how many ml of stock is needed?

6 ml

To make 2L of 10mM Tris-HCl from a 0.5M stock, how many ml of stock is needed?

40 ml

To make 1ml of 1X BSA from a 10X stock, how many µl of stock is needed?

100 ul

1L of 0.5% SDS solution contains how many grams of SDS?

5g

2L of 70% ethanol contains how many ml of ethanol?

1,400ml

Concentration of resulting solution: 300ml of 20mM NaCl mixed with 200ml of 120mM NaCl?

60mM

Primers: You have 19.5 nmoles of powder. To make a 75 µM solution, how much water do you add?

260 ul

DNA Reconstitution: You have 23 μg of DNA powder. To make a 100ng/μl solution, how much water is needed?

230 ul

RNA Reconstitution: You have 89 nmole of RNA. To make a 100 μM solution, how much water is needed?

890 ul

PCR Calc: A 25μl reaction contains 2μl of 10mM dNTP. What is the final concentration of dNTP in mM?

0.8 mM

Master Mix: For 11 reactions (10ul each, 1x buffer, 5% enzyme, 200ng DNA from 400ng/ul stock), how much water is in the master mix?

88 µl