micro lab quiz 2
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37 Terms
what type of agar is mannitol salt agar? MSA
selective and differential agar
what is the SELECTIVE agent in MSA?
salt
what is the DIFFERENTIAL agent in MSA?
mannitol
what is the ph indicator in MSA?
phenol red
name 1 bacterium GENUS and SPECIES that grows on MSA and uses the differential agent
staphylococcus aureus
what COLOR is the agar around the bacterium that grows on MSA and uses the differential agent?
yellow
name 1 bacterium GENUS and species that grows on MSA but does not use the differential agent
staphylococcus epidermis
what COLOR is a bacterium that grows on MSA but does NOT use the differential agent is msa?
pink/red
name 1 bacterium GENUS that does NOT grow on MSA?
streptococcus pyogenes
why doesn’t this bacterium grow on MSA?streptococcus pyogenes
not salt tolerant
what type of agar is phenyl ethyl agar ? PEA
selective agar
what is the SELECTIVE AGENT in pea?
phenylethyl alcohol
what bacteria group does PEA select for?
gram positive
what bacteria GROUP does PEA select AGAINST?
gram negative
name 1 bacterium GENUS that grows on PEA?
proteus vulgaris
name 1 bacterium GENUS that should NOT grow on PEA?
e.coli
what type of agar is blood agar?
differential agar
what are hemolysisn?
break down red blood cells
how are bacteria growing on a BA plate distuinguish from each other?
hemolysis pattern
what is BETA hemolysis?
complete hemolysis, clear zone
name a bacterium GENUS and SPECIES that is BETA hemolysis?
staphylococcus aureus
what is alpha hemolysis?
partial clearing in blood
name 1 bacterium GENUS and SPECIES that is alpha hemolysis?
streptococcus pneumoniae
what is gamma hemolysis?
no effect on blood
name 1 bacterium genus and SPECIES that is gamma hemolytic?
staphylococcus epidermis
what type of reaction is the staphytect reaction
agglutination reaction
what are the structures on the surface of a bacterium called?
antigens
what are the STRUCTURE in the staphytect REAGENT that respond to the structures on the bacterium?
antibodies
what does a POSITIVE reaction for the staphytect reaction look like?
clumping
what bacterium GENUS and species is positive for the staphytect reaction?
staphylococcus aureus
what does a negative reaction for the staphytech reaction look like?
no clumping
name 1 bacterium GENUS and species that is negative for the staphytect reaction?
staphylococcus epidermis
what is the SUBSTRATE for the catalase reaction?
hydrogen peroxide
what is the enzyme for the catalase reaction?
catalase
what are the products of the catalase reaction?
oxygen and water
name 1 bacterium GENUS that is positive for the catalase reaction?
staphylococcus
name 2 bacteria GENERA that are negative for the catalase reaction?
streptococcus and enterococcus