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What is the purpose of the promoter region of a gene?
It is a region of DNA where transcription factors like sigma bind to initiate transcription
Explain what is meant when we say genetic code is redundant or degenerate
Amino acids are 8 letter codes forming “codon” enter the LAST letter can be multiple things but still mean the same amino acid
Why don’t bacteria have to add 5’CAP and 3’ polyA tail to their mRNA molecule after transcription?
Eukaryotes-no nucleus/nuclearpore to move through
What happens to introns and exons during the splicing event?
Introns stay in the nucleus but exons get to exit with the mRNA
What RNA is transcribed but not translated
tRNA, rRNA, snRnA
A site-aminoacyl site (arrival)
Charged tRNA arrives to match the codon in mRNA. The aa is deposited with the ribosome, still attached to the tRNA
P site-peptidyl site (polypeptide)
Polypeptides are formed by combing aa with peptide bonds. The tRNA are patiently hanging around
E site-exit site (exit)
leaves the amino acid, the tRNA (uncharged now) is ready to exit the ribosome complex and be reused
Promoter
Transcription
Initiation factors
translation
P site
Translation
Regulatory elements
transcription
Splicing
Transcription
rRNA
Translation
ribosome
translation
Met
translation
tRNA
translation
amino acids
translation
codon
translation
TATA box
transcription
charged tRNA
translation
AUG start
translation
elongation factors
translation
anticodon
translation
A site
translation
sigma factor
transcription
poly A tail
transcription
terminators
transcription
5’CAp
Both
ribosome binding site
translation
template strand
transcription
The central dogma gene expression
During transcription DNA codes for mRNA, which codes for polypeptides during translation
Why don’t bacteria have to add a 5’CAP and 3’ polyA tail tot their mRNA molecules after transcription
They don’t experience the transport through the nuclear pore and fraying because there is not a membrane-bound organelle
A gene is
a specfic segment of nucleotides in DNA