Bio Lab 4

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Last updated 4:26 AM on 6/10/26
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7 Terms

1
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beers law

when a solute absorbs light of a particular wavelength, the absorbance is directly proportional to the concentration of the solute in solution.

shows linear relationship between absorbance and concentration (as one increases the other increases)

2
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most commonly used measurement for protein assays

absorbance of light

3
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Bradford assay

uses Coomassie brilliant blue

4
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Spectrophotometer

sends a beam of light through the sample, and measures how much of the light gets through

5
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absorbance

the amount of light absorbed, expressed in arbitrary units called absorbance units or optical density

6
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which spectrophotometer did we use

the Genesys 30

  • emits a broad range of wavelengths

7
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Protein composistion

Bovine globuline (BGG) dissolved in phosphate buffered saline (PBS)

(most common protein for standardization)