Micro Lab Practical

Least effective and most effective hand cleansers:

Green soap, hibiclens

Where microbes were most present

Floor, cough, fingertips

What whole colony shape is this?

Round

What whole colony shape is this?

Irregular

What whole colony shape is this?

Spindle

What whole colony shape is this?

Filamentous

What whole colony shape is this?

Rhizoid

What margin is this colony?

Smooth

What margin is this colony?

Irregular

What margin is this colony?

Lobate

What margin is this colony?

Filamentous

What margin is this colony?

Rhizoid

What is this colony’s elevation?

Convex

What is this colony’s elevation?

Umbonate

What is this colony’s elevation?

Plateau

What is this colony’s elevation?

Flat

What is this colony’s elevation?

Raised

What is this colony’s elevation?

Raised, spreading edge

What is this colony’s elevation?

Flat, raised margin

What is this colony’s elevation?

Growth into medium

What is this growth pattern?

Uniform fine turbidity

What is this growth pattern?

Flocculent growth

What is this growth pattern?

Pellicle

What is this growth pattern?

Ring

What is this growth pattern?

Sediment

Fluid thioglycollate broth (FTB) tests for what?

Aerotolerance

What reaction takes place in fluid thioglycollate broth (FTB)?

Oxidation of resazurin

What happens in an anaerobic jar?

CO2 increase, O2 becomes dehydroascorbic acid and water

Collective term for specie’s minimum, maximum, and optimal temperatures

Cardinal temperatures

Psychrophiles’ growth range in C

Below 20

Psychrotrophs’ growth range in C

0-30

Mesophiles’ growth range in C

15-45

Thermophiles’ growth range in C

Above 40

Extreme thermophiles’ growth range in C

Above 65

Acidophiles’ growth range in pH

Below 5.5

Neutrophiles’ growth range in pH

5.5-8.5

Alkaliphiles’ growth range in pH

Above 8.5

What is this organism?

Amoeba

What organism is this?

Aspergillis

What organism is this?

Penicillium

What organism is this?

Candida

Gram negative color

RedG

Gram positive color

Purple

Acid-fast stain is detect the presence of

Mycolic acids

Reagent used in cold kinyoun/acid-fast, decolorizes cells

Acid alcohol

What are those green dots?

Endospores

What is this flagella arrangement?

Polar

What is this flagella arrangement?

Amphitrichous

What is this flagella arrangement?

Lophotrichous

What is this flagella arrangement?

Peritrichous

Mannitol salt agar (MSA) is what type of medium?

Selective, differential

What organism does mannitol salt agar (MSA) differentiate for?

S. aureus

MacConkey agar is what type of medium?

Selective, differential

What ability does MacConkey agar differentiate with phenol red, a pH indicator?

Ability to ferment lactose

What is added to bacteria in a catalase test?

Hydrogen peroxide

What color appears in an oxidase test if what enzyme is present?

Purple, cytochrome c oxidase

Citrate utilization test differentiates members of what group?

Enterobacteriaceae

A blue result in a citrate ulitization test means what?

Positive, citrate is utilized

Clearing around growth in a starch hydrolysis test means what?

Positive, amalyse is present

What organism does the starch hydrolysis test identify?

G. vaginalis

What does a yellow result in a urea hydrolysis test mean?

Negative, urease is absent

What organism can be identified with urea hydrolysis?

H. pylori

Bile esculin agar (BEA) is what type of medium?

undefined, selective, differential

No darkening in a bile esculin test means what?

Negative, does not hydrolize esculin

A positive bile esulin test means the organism is in what group?

Group D Streptococcus or Enterococcus

What three activities does the SIM medium determine?

Sulfur, reduction, indole production, motility

What color would sulfur reduction cause in a SIM medium?

Black

In a SIM medium, what is used to indicate an indole-positive organism by showing up red?

Kovac’s reagent

In a