ENDOSPORE STAIN - LAB PRACTICAL

After you put your micorbe on your slide you do what?

Prepare and heat-fix three smears (must allow to dry prior to heat-fix).

Step 2:

Place narrow paper towel on each smear (not hanging over edges).

Cut paper towel into little cubes,

Step 3:

Flood with malachite green and steam over water bath for 5 minutes.

For the LAST MINUTE you do NOT continue to add malachite green onto your slide.

Step 4 is what:

Keep stain moist; replenish if needed. DO NOT let water turn green.

(Malachite green is not safe! Do not breath and do not put down sink!)

Step 5:

You cool, then rinse gently with distilled water.

Then you counterstain with SAFRANIN for 2 minutes!!!!!!

Step 6:

After you rinse and blot dry.

Examine under oil immersion.