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How are cell membranes made?
Phospholipids self-assemble into a bilayer due to hydrophilic heads and hydrophobic tails.
What are the two protein sorting pathways?
Cytosolic and Secretory
What proteins are made in secretory pathway?
secreted proteins, membrane proteins, proteins for organelles
What is co-translational translocation?
Protein enters ER while being synthesized by ribosome
What crosses the lipid bilayer easily?
Small nonpolar molecules (O2, CO2)
What crosses lipid bilayer slowly?
Small polar molecules (water)
What cannot cross the lipid bilayer?
Ions and large polar molecules
What is diffusion?
Movement from high to low concentration
What is a concentration gradient?
Difference in concentration across space
What is a permease?
Membrane protein that helps transport substances
ΔG when moving down a gradient?
Negative (spontaneous)
ΔG when moving against gradient?
Positive (requires energy)
What is facilitated diffusion?
Passive transport using proteins
ΔG of facilitated diffusion?
Negative
Two types of permeases?
Channel proteins and carrier proteins
What is active transport?
Movement against gradient using ATP
ΔG of active transport?
Positive
What protein is used?
Protein pump (ATPase)
What is cotransport
One solute moves down a gradient to power another moving up
Types of cotransporters?
Symporters and antiporters
Two factors of electrochemical gradient?
Concentration gradient and electrical gradient
What is an aquaporin?
Water channel protein
Is there a water pump?
no
Endocytosis
Taking material into cell
Exocytosis
Releasing material out of cell
Main component of a plant cell wall?
Cellulose
Function of a cel wall?
Support and protection
Main component of a bacterial cell wall?
Peptidoglycan
Animal ECM main components?
Collagen, proteoglycans, fibronectin.
What anchors ECM to cells?
Integrins
Two main roles of epithelia
Protection and absorption/secretion
What keep membrane regions separate?
Tight junctions
Homeostasis
Maintaining stable internal conditions
Osmosis
Water movement across membrane
Isotonic
Equal solute concentration
Hypotonic
Lower solute outside —> water enters cell
Hypertonic
HIgher solute outside —> water leaves cell
Water potential Ψ
Measure of water movement
Solute potential Ψs
Effect of solutes - always negative
Pressure potential Ψp
Physical pressure on water
Ideal condition for plants
Hypnotic (turgid)
Salt-in method
Accumulate ions
Compatible solutes
Organic molecules (eg. sugars)
Central vacuole function
Stores water, maintains pressure
Stomata
Openings for gas exchange
Guard cells
Control stomata using water pressure
Contractile vacuole
Pumps out excess water
Freshwater protists need osmoreg?
Because water constantly enters cell
Breakdown of amina acids produces..
Ammonia
Three waste types
Ammonia, urea, uric acid
Most toxic waste
Ammonia
least water needed
Uric acid
Filtration in the kidneys occurs in
Bowman’s capsule
Reabsorption is in
Proximal tubule
Loop of Henle
concentrates urine
function of ADH
Increases water absorption —> concentrated urine.