PAG 1: microscopy - calibrating the graticule
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8 Terms
what is the eyepiece graticule
a small glass scale with 100 divisions and no units - which is fitted into the microscope eyepiece
what is the stage micrometre
a glass microscope slide with a scale measured in micrometres (µm) - which is placed onto the microscope stage
which is smaller: the eyepiece graticule or the stage micrometre
eyepiece graticule is smaller
why does the eyepiece graticule have to be calibrated
so that the arbitrary units of the eyepiece graticule are converted into real life measurements (micrometres) so that cell structures can be measured
what is used to calibrate the eyepiece graticule
stage micrometre is used to calibrate the value of graticule divisions - enabling you to work out what what one graticule division is in micrometres at different magnifications
when does the eyepiece graticule have to be recalibrated
when changing magnification the graticule must be recalibrated because as magnification increases, the value of one graticule division decreases
» therefore must recalibrate for each objective lens as it will have a different magnification
what is the standard value of one stage micrometre division
10 µm
outline how to calibrate an eyepiece graticule
place eyepiece graticule into eyepiece
place stage micrometre onto stage
line up the scales together
count number of graticule divisions that fit into one stage micrometre division (1 micrometre division =10µm)
calculate the value of one graticule division by:
