124 RBC Weekend Lab

normal hematocrit values: newborn

53 - 65%

normal hematocrit values: adult female

37 - 47%

normal hematocrit values: adult male

42 - 52%

normal hemoglobin values: newborn

17 - 23 g/dL

normal hemoglobin values: adult female

12 - 16 g/dL

normal hemoglobin values: adult male

14 - 28 g/dL

MCV - mean corpuscular volume (fL)

measuring the size of the cell

normocytic MCV

80 - 100 fL

microcytic MCV

<80 fL

macrocytic MCV

>100 fL

MCV formula

hematocrit x 10 / # of RBCs (in millions)

MCH - mean corpuscular hemoglobin (pg)

measures the avg. weight of hemoglobin in RBCs

normal MCH

28 - 31 pg

MCH formula

hemoglobin x 10 / # of RBCs (in millions)

MCHC - mean corpuscular hemoglobin concentration (%)

measures overall assessment of Hgb

MCHC formula

hemoglobin x 100 / hematocrit

RDW

red cell distribution width (analysis of anisocytosis)

normal RDW

11.5 - 14.5%

anisocytosis

varying sizes of RBCs

normal ESR values: adult male

0 - 22 mm/hr

normal ESR values: adult female

0 - 29 mm/hr

RBC counting formula

(# of RBCs counted on grid x 10 x 200) / (0.2 × 0.2 × 0.5) = # of RBCs x 10⁶/uL

normal RBC values: newborn

4.4 - 5.8 × 10⁶/uL

normal RBC values: adult female

4.2 - 5.4 × 10⁶/uL

normal RBC values: adult male

4.7 - 6.1 × 10⁶/uL

WBC counting formula

(# of WBCs counted on grid x 10 x 20) / (1 × 1 × 4) = # of WBCs x 10³/uL

WBC normal values: newborn

9.0 - 30.0 × 10³/uL

WBC normal values: child/adult

4.8 - 10.8 × 10³/uL

platelet counting formula

(# of platelets counted on grid x 10 x 20) / (1 × 1 × 1) = # of platelets x 10³/uL

normal platelet values: child/adult

150,000 - 450,000 plts/uL

normal erythrocytes

• 6 - 8 microns in size

• biconcave shape

• central pallor

normochromic

contains normal amount of Hgb

hypochromic

contains less Hgb than normal

hyperchromic

contains more Hgb than normal

polychromasia

not fully matured RBCs that contain RNA remnants; appear purple

name the type of RBC

target cell

name the type of RBC

spherocyte (loss of biconcave shape)

name the type of RBC

ovalocyte

name the type of RBC

elliptocyte

name the type of RBC

mouth cell/stomatocyte

name the type of RBC

sickle cell/drepanocyte

name the type of RBC

spiny cell/acanthocyte

name the type of RBC

burr cell

name the type of RBC

torn RBC/schistocyte

name the type of RBC

helmet cell

name the type of RBC

tear drop cell

name the type of RBC inclusion

howell-jolly bodies

name the type of RBC inclusion

basophilic stippling

name the type of RBC inclusion

pappenheimer bodies

name the type of RBC inclusion

heinz bodies

name the type of RBC inclusion

hemoglobin C crystals

name the type of RBC inclusion

cabot ring

variations in RBC distribution: agglutination

• clumped RBCs

• caused by cold acting antibodies

variations in RBC distribution: rouleaux

• RBCs stacked on top of each other

• caused by increased protein in blood

hematocrit determination (manual)

measurement of packed RBC volume

hemoglobin determination (automated)

RBC + lysing agent = free Hgb

erythrocyte sedimentation rate (ESR)

non-specific assay to screen for INF

osmotic fragility test

assay used to analyze integrity of the RBC membrane

fecal occult blood test

assay used to detect free Hgb in stool

sickledex

tube solubility screening for Hgb S

iron prussian blue stain

kleihauer-betke (fetal hemoglobin) stain

heinz body stain

reticulocyte stain