CP13: isolate and individual species from a mixed cutlure of bacteria using streak plating

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Last updated 8:55 AM on 4/23/26
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9 Terms

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use of aseptic technique example

● Wipe down surfaces with antibacterial cleaner both before and after experiment.

● Use a Bunsen burner in the work space so that convection currents draw microbes away from the culture.

● Flame the inocculating loop and allow TO COOL before using to transfer bacteria.

● Flame the neck of any bottles before use to prevent any bacteria entering the vessel (air moves out so unwanted organisms don’t move in).

● Keep all vessels containing bacteria open for the minimum amount of time.

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<p>how to streak plate </p>

how to streak plate

  • streaks must overlap

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method

  1. flame inoculating loop to sterilise (until it glows red) and allow to cool

  2. flame neck of mixed culture tube to sterilise

  3. dip inoculating loop into mixed culture

  4. flame neck of mixed culture tube agai and replace lid

  5. open the petri dish lid as little as possible - streak plate

  6. flame inoculating loop again

  7. tape the lid of petrish dish (not fully to prevent anaerobic growth) and leave to incubate eg. for 48 hours

  8. after incubation take a sample of a red colony using and inoculating loop and repeat the process of a fresh nutrient agar plate

  9. take a sample of a yellow colony and do the same on a fresh nutrient agar plate

  10. tape the lids of petri dishes (not fully to prevent anaerobic growth) and leave to incubate

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why do you not fully petri dishes

to prevent anoxic/anaerobic conditions so that harmful pathogens don’t grow

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incubate at temperature below body temp at 25C

to prevent growth of human pathogens

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why do you incubate petri dishes upside down

to prevent water condensation on the lid from dripping onto the agar surface and colonies mixing

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risks

  • flammable disinfectant

  • biohazard - contamination infection

  • naked flame - fire hazard/burns

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how to minimise risk

  • keep away from naked flame

  • aseptic technqiue, washing hands

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topics it links into

plate → broth

antibiotics → spread plates → zone of inhibitions

gram positive and negative bacteria