information for bis M1

At pI, the isoelectric point, what is true about a molecule?

It will not

1- move in an electric field

2-bind to an ion exchange column

3- bind to another molecule electrostatically

pH = what? (In terms of pOH)

pH= 14 - pOH

Formula for pI

Acid and base proportion equations (a/b)

Which Ionizable AA are +/0 and which are 0/- ?

HH equation

pH= and pOH= (basic concentration conversion)

Ka and Kb in terms of pKa and pKb

How to calculate pH for phosphate buffers b

Ka and Kb (x equations)

[H] or [OH] in terms of pH and pOH

Statements about hydrophobic effect

-water forms clathrates around a hydrophobic compound

-decreasing the temperature caused water molecules to become more ice like

-the hydrophobic effect allows cells to have compartments

-the hydrophobic effect causes the entropy of water to increase

False statements about hydrophobic effect

-increasing temp diminished hydrophobic effect (opposite is true)

-pH and direction matter

-distance matters

-the hydrophobic effect does not allow proteins to fold correctly

-water molecules form strong covalent bonds around the hydrophobic compound

What does one peptide bond plane look like in an oligopeptide?

Why are atoms in a peptide bond in a plane shape?

Their resonance forces the peptide bond into a plane shape, as the double bond at the C=O can also resonate to become C=N. The peptide bond has a 40% double bond character

True statements about Myoglobin

-When O2 binds to it, the metal become planar relative to the porphyrin ring

-the proximal histidine forms a covalent bond due to the metal in the heme

-myoglobin has a very low P50 when assayed in vitro

-when Mb is in solution, its interior is dynamic

False stamens about myoglobin

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