Benedicts Solution

What does Benedicts solution test for?

Reducing sugars (broken up carbohydrates)

How big should the test tube sample be?

5 cm cubed

How to get the sample?

• Crush food item with mortar and pestle

• Stir it in a beaker with distilled water until mostly dissolved

• Strain it through a funnel lined with filter paper

• Filtered into a test tube to finish creating the sample!

What is the second step of the experiment?

Prepare a water bath (75 degrees celsius)

What is the third step of the experiment?

Add 10 drops of Benedict’s solution to test tube

What is the fourth step of the experiment?

Place the test tube in the water bath for 5 minutes, using a using a test tube holder

What should the sample look like if there are no reducing sugars present?

It should stay blue.

How should the sample look if there are some reducing sugars?

In low concentration, (0.5-1%), the solution should turn yellow.

How should the sample look if there are trace amounts, or very little amounts of reducing sugars?

If there are trace amounts (0.1-0.5%), the solution should turn green.

How should the solution look if there is a moderate concentration of reducing sugars?

If there is a moderate concentration, (1-1.5%) the solution should be orange.

How should the solution look if there is a high concentration of reducing sugars?

If there is a high concentration (>2%), the solution should be brick red.