Chromatography and Radioisotopes

These flashcards cover the key concepts, methods, and terminology from the chromatography and radioisotope lecture notes.

Chromatography is used for _ of small weight molecules.

separation and analysis

The ___ action drives the process of chromatography.

capillary

In paper chromatography, the stationary phase is __.

water

The mobile phase in paper chromatography can be a variety of __.

solvents

In paper chromatography, separation occurs primarily due to differences in __.

polarity

The solid support in thin layer chromatography (TLC) can include substances like silica gel or __.

aluminum oxide

The advantages of thin layer chromatography over paper chromatography include greater __, speed of separation, sensitivity, and versatility.

resolution

Elution in chromatography can occur by a single solvent, stepwise, or __.

gradient

In affinity chromatography, separation is based on highly specific interactions such as __ and antibodies.

antigen

Gel filtration chromatography separates molecules based on __ and shape.

size

Ion exchange chromatography uses a solid stationary phase made of resin with attached or groups.

cationic, anionic

Chromatofocusing separates proteins exclusively using their __ point.

isoelectric

Hydroxyapatite chromatography uses CaPO4 crystals to bind with __.

DNA, RNA, Chromatin

HPLC stands for __ liquid chromatography.

high-performance

The actual measurement of radioactivity is given by disintegrations per minute (dpm), while counts per minute (cpm) is an __.

estimation

Three types of radioisotopes include alpha particles, beta particles, and __ particles.

gamma

Gamma rays have the greatest penetrating power and the __ ionizing power.

lowest

The simplest unit to measure radioactivity is __.

Curie (Ci)