DNA Extraction

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Last updated 3:14 PM on 2/6/24
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4 Terms

1
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steps in DNA extraction

  1. collection

  2. burst cells open to release DNA

  3. separate DNA from proteins and debris

  4. isolate concentrated DNA

2
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components of lysis solution and their uses

(1) detergent - disrupts cell membrane and nuclear envelope, causing the cells to burst open and release their DNA

(2) proteinase K - curs histones to free DNA

3
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purpose of salt solution

cause proteins and other cellular debris to clump together

4
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purpose of isopropyl alcohol

cause DNA precipitation