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What are proteins made of
amino acids
Essential amino acids
Indispensable: NOT made in body
Nonessential amino acids
Made in liver/other tissues
Conditionally indispensable amino acid
NOT made in body during stress
6 of the dispensable amino acids fall into here
Major protein roles for the body
tissue growth, muscle repair, adaptation, immune function

Structural, enzymatic, transport, and repair roles

What happens to excess protein
too much → not stored, used as energy or fat
When protein is used for energy
during endurance exercise, when glycogen is low
Protein recommendations for athletes in g/kg
Recreational athlete: Inactive person in recreation activity may increase protein intake from 0.8 to 1.0 g/kg/d
Middle-distance runner: may increase protein intake from 1.2 to 1.5 g/kg/d (cuz volume increase)
Active muscle-building athlete: may increase protein intake to 2.0 g/kg/d to support skeletal muscle mass
Why athletes require more protein than sedentary individuals
Athletes build muscle protein through training, which requires more protein

Importance of protein timing
Moderate amount of protein 3 times a day stimulates muscle protein synthesis to a greater degree
General protein intake per meal
Maximum dose 20-30 grams per meal
Define nitrogen balance
Difference between muscle protein synthesis and muscle breakdown
Positive nitrogen balance
growth period, hypertrophy, bigger muscles
Calculate protein needs based on body weight
Step 1: Nitrogen intake
Step 2: Nitrogen Output
Step 3: Nitrogen balance

Negative nitrogen balance
Muscle loss, breaking down protein
Nitrogen output calculation
UUN (aka urinary urea nitrogen) + 4
Nitrogen balance calculation
nitrogen intake - nitrogen output = Nitrogen balance
How many kcals from protein?
4 kcals
Nitrogen Intake Calculation
Grams of protein / 6.25