Microscopes

Resolution

The ability of a microscope to show detail.

Body tube

Part of the microscope that maintains the proper distance between lenses.

Ocular lens (eye piece)

Component through which the observer looks.

Revolving nosepiece

Allows the selection of different lenses.

Objectives

Lenses providing varying levels of magnification.

Stage clips

Holds the slide in position.

Diaphragm

Regulates the amount of light passing through the microscope.

Light source

Illuminator that produces light for the microscope.

Arm

Supports the body tube of the microscope.

Stage

Platform that supports the slide being viewed.

Coarse adjustment knob

Adjusts the stage significantly for focusing.

Fine adjustment knob

Adjusts the stage slightly for focusing.

Base

The bottom part that supports the entire microscope.

Light microscope

Uses glass lenses to magnify objects with light.

Scanning Electron microscope

Utilizes a beam of electrons to examine the surface of objects.

Transmission electron microscope

Produces 2D images by passing electrons through thin slices of specimens.

Total magnification

Calculated by multiplying the magnification of the ocular lens by the objective lens.

Diameter of the field of view

The diameter of the area visible through the microscope at different magnification levels.

Advantage of light microscope

relatively cheap and easy to use, you can see living things and life processes at work like fluid uptake and movement.

Disadvantage of light microscope

They only magnify so much-  at most 1000x

Advantage of scanning electron microscope

You can see great detail of the outside of an object. It can magnify a lot up to 500,000x or more.

Disadvantage of scanning electron microscope

They are very expensive, you need special training, and the organism is killed

Advantage of scanning electron microscope

you can see great detail of the inside of an object. it can magnify a lot up to 500,000x or more

Disadvantage of scanning electron microscope

They are very expensive, you need special training, and the organism is killed

Low Power    10 x 4

40x

Medium Power    10 x 10

100x

High Power    10 x 40

400x

Field of view

Low power

4.5mm

Field of view

Medium power

1.8 mm

Field of view

High power

0.45 mm