Safety and Lab Equipment Review

Chemical Hygiene Plan

CHP stands for....

a safety doc. that outlines procedures, equipment, and practices

CHP is....

Safety Data Sheet

SDS stands for...

A detailed doc. that provides info about a chemical substance or product

SDS is...

Occupational Safety Health Administration

OSHA stands for...

Sets and enforces standards that ensure the safety of work environments

OSHA does what?

US Department of Labor

OSHA is apart of what US Department

Personal Protective Equipment

PPE stands for...

Gear/clothing designed to protect staff from work place hazards

PPE is...

Any biological substance that poses threat to living organisms

What is a biohazard?

Anything that poses a danger/risk to health/safety in an environment

What does hazardous mean?

Infectious DZ in the bloodstream or other bodily fluids

What is a bloodborne pathogen?

A DZ transferable from animals to humans

Zoonotic DZ is....

Scabies

An example of a Zoonotic DZ is...

Controls used to eliminate/reduce work exposure by changing the work environment

What are engineering controls?

Vet hoods

An example of an engineering control is...

Specific protocalls to minimize worker exposure

What are administrative controls?

CHP

An example of administrative controls is...

Policies that set standards for worker behavior

What are procedural controls?

Sharps Management Procedures

An example of procedural controls is...

Got something from a hospital or something similar

What is a Nosocomial DZ?

Kennel Cough

An example of Nosocomial DZ is....

Injury resulting from a treatment

What does Iatrogenic mean?

a patient getting hair loss following their chemo treatment

An example of iatrogenic would be....

4

How many levels of biosafety is there?

Affects people with low immune systems

Biosafety Level 1

1

Infectious Canine Hepaitis is an example of which biosafety level?

DZ cause from oral ingestion, mucous membrane exposure, and skin puncture

Biosafety level 2

2

Salmonellosis and Taxoplasmosis is an example of which biosafety level?

Serious/possible lethal DZ

Biosafety Level 3

3

Mycobacterlum Tuberculosis is an example of which biosafety level?

Life threatening, very likely to be aerosol

Biosafety Level 4

4

Ebola is an example to which biosafety level?

Seperate

The veterinary clinical laboratory should be located in an area that is ______ from other hospital operations.

How much light bends/refracts as it passes through a substance

Refractive Index

Substance/compound added to test for the presence of another substance

Reagent

Total solids

TS stands for....

Total Protein

TP stands for...

Packed Cell Volume

PCV stands for...

Sodium Citrate

Blue Cap color

No additives

Red/White cap color

Heparian

Green cap color

EDTA

Purple cap color

Gel separator and clot activator

Red/Gray, gold or Red/Back (Tiger Top) cap color

Blue

Which cap color's primary use is for coagulation studies?

Red/White, red/gray, gold, and tiger top

Which cap color's primary use is for serum in blood chemistry?

Green

Which cap color's primary use is for plasma for blood chemistry?

Purple

Which cap color's primary use is for hematology?

Gray

Which cap color's primary use is for coagulation testing and glucose testing?

Potassium oxalate or sodium fluoride

Gray cap color

Red blood cells

What is number 4 called?

Plasma

What is number 2 called?

Buffy coat, WBC

What is number 3 called?

Wax coat

What is number 5 called?

The liquid portion of a spun down sample at the top

What is the supernatant?

The solid portion of a sample, at the bottom

What is the sediment?

Microhematocrit Centrifuge

What type of centrifuge spins down hematocrit tubes?

Angled and Horizontal (Swinging arm)

What 2 centrifuges did we cover the most in class?

Balanced

A centrifuge must always be ______ with tubes of equal size and weight

False

T or F: Centrifuges separate substances pf different volumes

RPM

What are centrifuges measured in?

Total protein of plasma/serum and Specific gravity of urine samples

What two things does a refractometer measure?

g/dL

What units does the refractometer use?

A pipette with no specific measurements

Transfer Pipette

A pipette with specific measurements

Graduated Pipette

37

Incubators must be capable of sustaining a constant _____ degree Celsius

52

Angled centrifuges are fixed at a ______ degree angel

To keep samples at a constant temp

What are water baths/heat blocks used for?

To prevent blood from cloting

What is a blood rocker used for?

Aliquot Mixer

What is another term/name for blood rocker?

4x

What power is the objective lens: Scanning?

10x

What power is the objective lens: Low Power?

40x

What power is the objective lens: High Dry?

50x

What power is the objective lens: Low oil?

100x

What is the objective lens: Immersion Oil?

Cloting

Coagulation means...

Soloxine

On the first notes day, what medication did the Vet Tech fill in the video?

Fine

Which focus knob can help you see the edges of the slide better?

x10

What magnification is the Ocular lens usally?

400

x10 (ocular) x40 (high dry)= 400

If you are looking through a normal microscope ocular lens of x10, and the high dry lens, what is the total magnification?

The objective lenses

What does the nose piece on a microscope hold?

general focus

The course focus knob is the....

sharp focus

The fine focus knob is the....

raises or lowers to focus the light to the slide

The substage condensar...

Controls the amount of light on the slide

The aperture diaphragm....

Occular Lens

What is A called?

Diopter Adjustment

What is B called?

Arm

What is C called?

Coarse Focus

What is D called?

Fine Focus

What is E called?

on/off switch

What is F called?

Nose Piece

What is G called?

Objective Lens

What is H called?

Slide Holder

What is I called?

Stage

What is J called?

Condenser

What is K called?

Aperture Diaphragm

What is L called?

Light Source

What is M called?

What is N called?

What is N called?