Cell Lines Quiz

Colon, colorectal adenocarcinoma

Caco-2 cells originate from which organ and disease?

Human

HaCaT cells come from which species?

Kidney

HK-2 cells originate from which organ?

Macrophages (mouse)

RAW 264.7 cells model which immune cell type?

Human

HEK293 cells come from which species?

Suspension (T cells)

Are Jurkat cells adherent or suspension?

Human

What is the species of origin of Jurkat cells?

Fibroblast (NIH/3T3, mouse embryo)

What is the cell type of NIH/3T3: fibroblast or macrophage?

mESCs (mouse embryonic stem cells)

Which cell type on our list is pluripotent and grows in round colonies?

Human Embryonic Kidney

What does "HEK" in HEK293 stand for?

Mouse (teratocarcinoma)

P19 cells come from which species?

Mouse

RAW 264.7 cells come from which species?

Keratinocytes

HaCaT cells are immortalized cells of which skin cell type?

Liver

HepG2 cells originate from which organ?

African green monkey

Vero and COS-7 cells come from the kidney of which animal?

Adherent

Are HeLa cells adherent or suspension?

Breast adenocarcinoma

MCF-7 cells come from which type of cancer?

Colon, colorectal carcinoma

HCT116 cells come from which tissue and disease?

Adherent

Are MCF-7 cells adherent or suspension?

HeLa

Which cell line is famous both for being the first immortalized human line and for its historic role in developing the polio vaccine?

Vero

African green monkey kidney; cannot make interferon; established in Chiba, Japan, 1962.

HeLa

HPV-18 DNA in the genome; adherent and double about every 24 hours; first immortal human cell line from Henrietta Lacks

HepG2

15-year-old male donor; initially called hepatocellular carcinoma, now hepatoblastoma; liver model with a doubling time of about 48 hours

MCF-7

isolated in 1970 from a pleural effusion; express estrogen and progesterone receptors; honors the Michigan Cancer Foundation; breast cancer line

HCT116

KRAS G13D mutation; mismatch-repair deficient (microsatellite unstable); adherent, epithelial-like human colorectal carcinoma line

Jurkat

make IL-2 when activated; grown in suspension in RPMI-1640; taken from a 14-year-old boy with T-cell leukemia

COS-7

CV-1 cells were transformed with origin-defective SV40; fibroblast-like cells; African green monkey kidney

RAW 264.7

Abelson murine leukemia virus-induced tumor; doubling time at 11-17 hours; mouse macrophage-like line used to study inflammation

NIH/3T3

focus-formation assays rely strong contact inhibition; fibroblast; Swiss mouse embryp

HaCaT

spontaneously immortalized from the skin of a 62-year-old man; extracellular calcium controls differentiation; human keratinocyte line

P19

retinoic acid turns it into neurons; DMSO turns it into cardiac and skeletal muscle; from mouse teratocarcinoma

Caco-2

Which line forms polarized, enterocyte-like cells with a brush border after ~21 days?

SV40

COS-7 was made by transforming CV-1 cells with a defective form of which virus?

Hepatoblastoma, 15-year-old male

HepG2 is now known to be which disease, from what donor?

estrogen receptor (ER) and the progesterone receptor (PR)

Which two hormone receptors does MCF-7 express?

HCT116

Which line is MMR-deficient (MSI) with KRAS G13D?

HPV-16 E6/E7

Which viral oncogenes immortalized HK-2?

IL-2

Which cytokine do activated Jurkat cells produce?

RAW 264.7

Which line came from a tumor induced by Abelson murine leukemia virus?

~12 h (11-17 h)

RAW 264.7 doubling time: about 12 h, 36 h, or 72 h?

Calcium

HaCaT differentiation is controlled by which extracellular ion?

Caco-2

from colon cancer; act like small-intestine enterocytes; the standard model for drug absorption

NIH/3T3 and RAW 264.7

Which two mouse-derived lines here are fibroblast-like or macrophage-like adherent cells?

mESCs (~12-14 h), Jurkat (~24 h), HepG2 (~48 h)

Rank HepG2, Jurkat, and mESCs from shortest to longest doubling time.

P19

Which line gives cardiac/skeletal muscle with DMSO and neurons with retinoic acid?

LIF + feeders or 2i

Which additives keep mESCs undifferentiated?

Caco-2

Which line needs ~20% FBS and is used in drug-permeability assays?

Pleural effusion

MCF-7 was isolated from which body fluid?

Strong contact inhibition

Why is NIH/3T3 useful in focus-formation assays?

HPV-18

Which HPV type is integrated in the HeLa genome?

Vero

Sudden death: which line was established in 1962 in Chiba, Japan?

HEK293

from an embryonic kidney despite behaving like a neuron; genome is Adenovirus 5 DNA; favorite for transfection and making recombinant proteins