ABG Sampling

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Last updated 12:23 AM on 10/13/26
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28 Terms

1
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what are indications for arterial sampling

  • assess patient ventilation, oxygenation or acid base status

  • establish baseline values

    • documented lung disease requiring treatment

    • cardiopulmonary requiring surgery

  • clinical deterioration

  • response to therapy


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different artieral puncture sites ADULTS


  • radial artery

  • brachial artery

  • fermoral artery

  • doraslis pedis artery


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puncture sites for neonates

  • radial artery

  • superficial artery

  • umbilical artery (open for 24-48hr)


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advantages of radial artery

  • collateral blood flow to hand via ulnar artery

  • artery is superficial and easy to palpate and stabilize

  • less tissue to penetrate in this area and this means less pain

  • wrist is easily accessible

  • major nerves not in close proximity


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disadvantages of radial arterial sampling

  • radial artery more peripheral more likely to spasm

  • draw venous sample form veins

  • may be more difficult to obtain sample in poor circulation


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absolute contraindications

  • patient refuse

  • puncture site

    • Presence of a surgical shunt

    • Presence of localized lesions

    • Evidence of localized infection

  • Specific to femoral punctures:

• Do not perform outside of the hospita


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what is absolute contraindication for arterial sampling

poor collateral circulation

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relative contraindication for arterial sampling

• Deformities at puncture site area

• Raynaud's Disease

• Fibrosed arteries

• Hematoma at the puncture site

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relative contraindications for arteiral sampling

• Anticoagulant or thrombolytic medication

• A patient with a coagulation disorder (i.e. Hemophilia)

• Low platelet count or prolonged bleeding time

• A patient with severe hypotension

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complications of arterial sampling

  • Hematoma, hemorrhage

• Infection

• Thrombosis

• Arterial spasm

• Laceration of blood vessels or nerves

• Vasovagal response

• Pain

• Loss of limb, hand or fingers (rare)

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hazard for art smaplin

to health care workers

• Needle Stick Injury

• Blood borne infections

  • Human immunodeficiency virus (HIV)

    • Hepatitis B (HBV)

    • Hepatitis C (HC


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brachial puncture indications

radial puncture is no possible

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disadvantage of brachial puncture

Deeper than Radial Artery

Proximity of Median Nerve

No collateral circulation

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femoral artery sampling indications

Low perfusion

• Cardiac arrest/shock

• Deformities of the wrist

• Failed bilateral modified Allen’s test

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absolute contraindications for femoral artery sampling

  • femoral arterial grafts

  • trauma to the pelvic area


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advantages of femoral sampling

Less subject to low pressure problems

• Large diameter

• Less likely to go into spasm

• Easy to locate and puncture, however it is a deeper artery

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disadvantages of femoral sampling

Obstruction of the femoral artery greatly decreases blood flow to the leg

• Some collateral circulation via the deep femoral artery

• More susceptible to infection and hematoma

• Thrombosis (arteriosclerotic artery)

• Easy to puncture the vein

• Femoral nerve lies Immediately lateral to the artery

• Artery is less accessible and lies deep under the skin

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ABG sampling equipment

PPE

• Arterial blood gas sampling

• Plastic syringes with needle

• Caps & Needle guards

• Anticoagulant

• Plastic vs Glass syringes

• Chlorhexidine or alcohol swabs

• Sterile gauze

• Label

• Watch, Pen, Notepad

• SpO2 Probe

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characteristics of plastic syringes

Most are vented

• Most are prefilled with dry heparin

• Much more available than glass syringes

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plastic syringe vents are located where

syringe lunger

integral media allows only the air to escape through syringe

media then expands and seals when contracted by blood

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disadvantages of plastic syringes

If the syringe is not vented, the barrel may need to be pulled back to get blood

sample (mask the arterial pulse)

• May allow diffusion of room air into syringe (especially if iced)

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glass syringes characteristics

Plunger (barrel) moves freely, less friction than plastic

• The arterial pressure moves the plunge

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disadvantages of glass syringes

Tendency for air leak around the plunger

• The barrel will fall out with poor handling of the syringe

• Need specific needles for the syringes (some types)

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vacuum tubes points

  • used but must be filled completly

    • not common method to obtain an ABG


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anticoagulants

Lithium heparin – most common

• Sodium heparin (1000 IU/ml)

• Dry heparin is used in syringes in pre made arterial blood gas kits

• Oxalates and citrate anticoagulants should not be used as they alter the sample pH.

26
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when to do modified allens test

Done prior to radial artery puncture ONLY

• Normal test indicating collateral circulation - hand flush pink within 5-10 seconds

• Cannot be performed on critically ill patients who are uncooperative or unconsciou

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blood sample analysis should avoid these errors

Obtained anaerobically (no air bubbles)

• Properly anti-coagulated

• Analyzed within 30 minutes (less than 15 minutes is ideal)

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what infection control procedures should be followed

• During Procedure: Gloves, Face Protection (splash risk)

• After Procedure: Gloves as a minimum when handling any blood products

• Dispose and transport any blood products in biohazard bags or containers

• Sharps of any kind go in sealed biohazard containers

• Any blood spills should follow the hospital protocols for cleaning and disinfection