CLS 307 EXAM #3 STUDY GUIDE

What are the stages in the myelopoiesis maturation pool

Metamyelocyte, band neutrophil, segmented neutrophil

What are the stages in the Mitotic (Proliferative) Pool

Myeloblast, promyelocyte, myelocyte, metamyelocyte

What is a recurrent disease that contains giant lysosomal granules in the leukocytes

Chediak-higashi syndrome

All of the following are true, except?

Neutrophils and monocytes can not kill phagocytozied pathogens

Select what is true about Pelger huet anomaly

All of the above

Reilly bodies are differentiated against toxic granulation for the following EXCEPT

Toxic granulation occurs in all leukocytes, while reilly bodies occur only in segmented neutrophils

What is CD16 and CD56?

large lymphocytes AKA “Natural Killer Cells” (NK)

what are the CD markers for T cells and B cells

T cells: CD3+CD4+ B cells: CD19+CD20+CD3-

In the case study it has symptoms of hemolytic anemia, chills, fever, hepatosplenomegaly. What is the possible diagnosis?

infectious mononucleosis

True or false? This WBC can increase up to 50% during this disorder.

True

Judging from this case study, what is this caused by?

Binds to CD21 (C3d receptor) on B cell

What is a confirmatory test for this disease?

EBNA specific antigen and antibody test

Acid sphingomyelinase

Nieman pick cells

Associated with bacterial infection, acute alcoholism, autophagocytosis

Cytoplasmic vacuoles

Made of remnants of ribosomal RNA

Dohle bodies

Beta-glycocerebrosidase

Gaucher cells

Darkly staining cytoplasmic granules in neutrophils

Toxic granulation

Conducted flow cytometry of patient who was 67 yr old male. What does this indicate?

acute lymphoblastic anemia (ALL).

what stain would best fit platelets and megakaryocytes

Factor VIII antibodies

What stain is used for HCL or T-cell ALL

TRAP or acid phosphatase (ACP)

What stain can you find auer rods and myeloblast?

MPO

What is a stain used for lipids?

SBB

mutation in ITGB2 gene which encodes CD18 subunit of β2 integrins

LADI

Selectin deficiency (mutation in SLC35C1 gene)

LADII

mutation in Kindlin-3

LADIII

To prevent WB or BM samples stored for a long time with the anti-coagulants…

use within the first 4h after collection

To prevent Tissue samples (BM FNB, LNB) not transferred to the lab in appropriate media…

culture media or saline-moistened gauze)

To prevent using harsh vortex on cell pellets…

use special vortex designed for cell pellets, use in pulses, 2 is fine, and avoid harsh centrifugation, follow the company’s SOP

Using aggressive mechanical disruption of tissues will negatively affect the cell viability (T or F)

True

To prevent cell pellets are kept as dry for a long time…

resuspend quickly but very gently in the relevant buffer and avoid formation of bubbles as they can kill the cells

To prevent Highly concentrated cell suspension is used…

adjust cell number according to the protocol, dilute if needed)

Keeping cells on ice can improve the viability as this slows down necrosis and the cell metabolism (T or F)

True

It’s highly recommended that old samples (>48h or earlier if not stored at 4 °C) should not be analyzed (T or F)

True

Which of the following is NOT true about Leukocyte adhesion disorders (LADS)

LADIII is when mutayion in ITGB2 gene which encodes the CD18 subunit of beta intergins

May-Hegglin anomaly is a mutation in what gene?

MYH9