L20- recombinant technology 2

what is DNA ligase 

what are the important regions that are on plasmids for cloning 

describe what blue-white screening

what is the ideal characteristics for a host used for DNA coding 

describe the production of recombinant insulin 

what are three changes/ways to change the release profile of recombinant insulin

when are bacteria and yeast hosts commonly used 

small proteins that aren’t post translationally modified ( these are cheap, fast growth and high yield )

when are insect/mammalian cells or whole animals used as hosts for recombinant tech 

when proteins of mammalian origin that are large or glycosylated need to be produced ( these are more expensive slow and lower yield )