card 4 COMPETITIVE ELISA

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Last updated 6:00 PM on 10/2/26
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15. In competitive ELISA, what happens when the sample contains more antigen?

A. More free antibody remains

B. Less free antibody remains

C. No antibody binds

D. More color is always produced

Correct Answer: B. Less free antibody remains

🧠 Explanation: More antigen → more antigen-antibody complexes → less free antibody.

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16. In competitive ELISA, more antigen in the sample produces:

A. More color

B. Less color

C. No change

D. More DNA

Correct Answer: B. Less color

🧠 Explanation: Tani waa qodobka ugu muhiimsan: More antigen → less color.

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17. In competitive ELISA, less antigen in the sample produces:

A. Less color

B. More color

C. No color

D. No antibody binding

Correct Answer: B. More color

🧠 Explanation: Less antigen → more free antibody → more binding to plate antigen → more enzyme → MORE color.

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18. Which ELISA has an inverse relationship between antigen concentration and color?

A. Direct ELISA

B. Indirect ELISA

C. Sandwich ELISA

D. Competitive ELISA

Correct Answer: D. Competitive ELISA

🧠 Explanation: Competitive ELISA: antigen ↑ → color ↓; antigen ↓ → color ↑.