SMALL GRAM-NEGATIVE NON-FERMENTATIVE BACILLI

non-fermentative

Pseudomonas species are the most commonly isolated _______ bacilli.

motile

Pseudomonas species are obligately aerobic, non-spore-forming and _______.

BAP

Pseudomonas species grow on primary plated media (MAC and _______)

assacharolytic

Pseudomonas species are usually oxidizers with few _______ strains.

catalase

iochemical test results for Pseudomonas show positive reactions for Oxidase and _______.

K/K, -, -

On TSIA, Pseudomonas exhibits ____, (_) gas, and (_) H2S reaction.

mosselii

Pseudomonas fluorescent group includes P. aeruginosa, P. fluorescens, P. putida, P. veronii, P. monteili and P. _______.

luteola

Pseudomonas non-fluorescent group includes P. stutzeri, P. alcaligenes, P. pseudoalcaligenes and P. _______.

Blue

Pseudomonas aeruginosa is the agent of _______ pus.

42

Pseudomonas aeruginosa is a monotrichous organism that grows at _______°C.

Pseudomonas aeruginosa

On BAP, _________________ __________ colonies exhibit flat, blue-green or red or brown color with ground glass or metallic sheen appearance and "grape-like" or corn tortilla odor.

Pyomelanin

Pigments produced by Pseudomonas aeruginosa include Pyoverdin, Pyocyanin, Prorubin, and _______.

dihydrolase

Distinguishing characteristics of Pseudomonas aeruginosa include positive growth at 42°C, (+) Acetamide and citrate utilization, (+) Gluconate production, and (+) Arginine _______.

septicaemia

Pseudomonas fluorescens and Pseudomonas putida are linked to transfusion-associated _______.

42

Pseudomonas fluorescens and Pseudomonas putida grow at 4°C but not in _______°C

Gelatin

Differential test for P. fluorescens is a positive result for _______ hydrolysis.

rods

Microscopic morphology of Pseudomonas shows straight and slender Gram-negative _______.

Cetrimide

Culture media for Pseudomonas include BAP, CAP, MAC, Sellers agar, and _______ agar.

thioglycollate

Pseudomonas produces scum or a "floating layer" on the uppermost layer and sides of _______ tube.

Pseudomonas

The Oxidative-Fermentative (OF) test is used to differentiate Enterobacteriaceae from _______.

glucose

Substrate used in the OF Medium is 1% carbohydrate (_______).

Bromthymol

The pH indicator used in OF Medium is _______ blue.

Green

Uninoculated OF medium exhibits a _______ color.

Fermenter

A yellow color in both open and closed tubes indicates a _______

Oxidizer

A yellow color in the open tube but green/blue color in the closed tube indicates an _______ but nonfermenter

tetracyclines

Growth of P. aeruginosa is inhibited by aminoglycosides, carbapenems, and quinolones and resistant to sulfamethoxazole-trimethoprim and _______.

Broth

Recommended susceptibility test for P. aeruginosa is _______ microdilution.

bacilli

Acinetobacter is the 2nd most commonly isolated, non-fermentative, Gram-negative _______.

non-motile

Acinetobacter is an obligate aerobe and _______.

baumannii

Glucose-oxidizing, non-haemolytic strains of Acinetobacter are identified as A. _______.

lwoffii

Non-glucose-oxidizing, non-haemolytic strains of Acinetobacter are identified as A. _______.

haemolyticus

Non-glucose-oxidizing, haemolytic strains of Acinetobacter are identified as A. _______.

purple

On MacConkey (MAC) agar, Acinetobacter colonies exhibit a _______ color.

oxidase

Biochemical test results for Acinetobacter show (+) Catalase and (-) _______.

bacillus

Stenotrophomonas maltophilia is the 3rd most commonly isolated non-fermentative, Gram-negative _______.

Stenotrophomonas maltophilia

On BAP, colonies are large and glistening with lavender-green to light purple pigmentation and distinct "ammoniacal odor".

oxidase

Stenotrophomonas maltophilia is positive for Catalase, esculin, gelatinase, DNAse, and LDC and negative for ______.

mallei

Burkholderia species are obligate aerobes and motile by polar flagella EXCEPT B. _______.

selective

Selective media for Burkholderia cepacia complex include Bacitracin lactose agar (OFBL) and Burkholderia cepacia _______ agar

Burkholderia cepacia

On BAP, ___________ complex colonies exhibit a non-wrinkled yellow or yellow-green color with "soil-like odor".

Nitrate

Burkholderia mallei yields a positive biochemical result for _______ reduction.

bipolar

Burkholderia pseudomallei microscopy exhibits the presence of _______ bodies.

Burkholderia pseudomallei

On Ashdown medium with colistin, _______ colonies are dry, wrinkled, deep pink or purple color with "earthy or soil-like odor".

BAP

Haemophilus species are fastidious, non-motile, and facultatively anaerobic bacteria that do not grow on _______ agar.

NAD

Haemophilus species require growth factors X (hemin) and V (_______).

Haemophilus influenzae

On CAP, _________________ colonies are translucent, convex, tan-colored and mucoid with a "mousy" or "bleach-like" odor.

Haemophilus ducreyi

ells on Gram stain exhibit a "school of fish" arrangement.

Kovac's

Reagent used in the Porphyrin test (Delta-aminolevulinic acid test) is _______ reagent.

Neufeld-Quellung

is a rapid direct identification test for capsular antigen of H. influenzae

Kingella

The HACEK group includes Haemophilus, Aggregatibacter, Cardiobacterium, Eikenella, and _______.

MAC

HACEK group species cannot grow on _______ agar.

star

Aggregatibacter actinomycetemcomitans on BHIA exhibits colonies with "_______-shaped" centers after 48 hrs of incubation.

Rosette

Cardiobacterium hominis exhibits _______ formation with sticklike projections on microscopy.

Eikenella corrodens

____________ __________ colonies pit or corrode the agar and have a "sharp bleach" odor.

Brucella

are obligate aerobes, fastidious, intracellular parasites and a Class B bioterrorism agent.

sandy

Microscopic appearance of Brucella shows small coccobacilli arranged singly, in pairs or in short chains with a "_______ appearance"

Castañeda

Culture media for Brucella include BAP, TSA, and _______ medium.

160

A positive Serum Agglutination Test (SAT) for Brucella is indicated by a titer of ≥1:_______.

Bordet

Bordetella species are obligately aerobic, fastidious Gram-negative coccobacilli cultured on _______-Gengou agar.

silver

On Bordet-Gengou agar, Bordetella colonies appear smooth and glistening with a _______ color.

tularemia

Francisella tularensis is a very small coccobacillus that causes _______.

bipolar

Microscopy of Francisella exhibits Gram-negative bacilli with faint _______ bodies and requires acridine orange stain.

Pasteurella

species are small, straight or ovoid Gram-negative bacilli with "safety pin" or bipolarstaining appearance.

urease

Pasteurella multocida yields positive results for Oxidase, indole, ornithine decarboxylase, and _______.

Legionella pneumophila

is primary acquired through inhalation or exposure to contaminated water from faucets and public water stations.

BCYE

Selective culture media for Legionella include _______ with L-cysteine, ferric salt, and α-ketoglutarate

Capnocytophaga

microscopy displays Gram-negative rods to filamentous or spindle-shaped bacteria