AP Bio Citric Acid Cycle/Kreb's Cycle

Products and Enzymes per 1 pyruvate

What is the Purpose of Kreb’s Cycle

regenerate oxaloacetate and create electron shuttles to be used in the electron transport chain (ETC)

Where does the Kreb’s Cycle happen

Matrix of the Mitochondria (innermost part)

Pyruvate Oxidation

Pyruvate → ___

Pyruvate enters mitochondria where it releases ___. Remaining carbon fragment is oxidized transferring electron from ___ to ___. ___ is attatched to 2-carbon intermediate

Acetyl CoA

CO₂

NAD⁺ to NADH

Coenzyme-A

Acetyl-CoA combines with ____ → ____ using enzyme ____ ;

releases ___

Oxaloacetate → Citrate

Citrate Synthase

CoA-SH

Citrate → ____ with enzyme ____ ;

1 ___ removed but then added back in

Isocitrate

Aconitase

H₂O

Isocitrate → ____ with enzyme ____ ;

uses 1 ___ producing 1 ___

releases ___

alpha-Ketoglutarate

Isocitrate Dehydrogenase

NAD⁺ → NADH

CO₂

alpha-Ketoglutarate → ____ with enzyme ____ ;

uses 1 ___ producing 1 ___

releases ___

adds ___

succinyl-CoA

alpha-ketoglutarate Dehydrogenase

NAD⁺ → NADH

CO₂

CoA

Succinyl-CoA → ____ with enzyme ____ ;

uses 1 ___ producing 1 ___ which is driven by the transformation of ___ into ___

Succinate

Succinyl-CoA synthase

1 ADP → 1 ATP

GTP → GDP

Succinate → ____ with enzyme ____ ;

uses 1 ___ producing 1 ___

Fumarate

Succinate Dehydrogenase

1 FAD → 1 FADH₂

Fumarate → ____ with enzyme ____ ;

adds 1 ___

Malate

Fumarase

1 H₂O

Malate → ____ with enzyme ____ ;

uses 1 ___ producing 1 ___

Oxaloacetate

Malate Dehydrogenase

1 NAD⁺ → 1 NADH

Net total of cycle per 1 glucose molecule:

__ ATP

__ CO₂

__ NADH

__ FADH₂

2 ___

6 ___

8 ___

2 ___

Net total of cycle & pyruvate oxidation per 1 glucose molecule:

__ ATP

__ CO₂

__ NADH

__ FADH₂

4 ___

6 ___

10 ___

2 ___