denaturation

1. increased in temperature

kinetic energy increases, so the bonds vibrate more

2. bonds breaking

hydrophobic and hydrophilic interactions, then hydrogen bonds then ionic bonds then disulfide bridges

3. tertiary structure unravels

as the bonds holding the tertiary structure in place have broken, the structure unravels and loses its complementary active site

4. no enzyme-substrate complexes

enzyme-susbtrate complexxes cannot be formed as there is no active site for the substrate to bind to