Histone/DNA modifications

Activation marks

H3K9ac, H3K27ac, H3K4me, H3K36me

Repression marks

H3K9me, H3K27me, H2AK118/119ub, DNA methylation

What does HP1 do?

binds H3K9me, recruits H3K9-HMT, chromatin aggregation

What does H3K9-HMT do?

place new methyls on adjacent H3K9

What does TFIID do?

senses H3K9ac and stays close so it’s ready for transcription when it needs to occur

What does PRC2 do?

places methyl group on H3K27 and maintenance methylation on H3K27 after replication

What does PRC1 do?

read methylated H3K27, aggregate chromatin, ubiquitation of H2AK118/119

What does BRD4 do?

read acetylated H3K27, recruitment of P-TEFb to the transcriptional apparatus

What does P-TEFb do?

phosphorylation of serine 2 on Pol II CTD

What does TFIIH do?

phosphorylates serine 5 on Pol II CTD

What does phorylated serine 2/5 on the Pol II CTD do?

recruits NELF/DSIF, capping complex, PolyA complex, splicing complex, SET1, SET2

What does SET1 do?

methylating H3K4

What does SET2 do?

methylate H3K36

What does TrxG do?

methylate H3K4 and H3K36, recruit CBP, maintains H3K4 and H3K36 methylation after replication

What does CBP do?

acetylates H3K27

What does UHRF1 do?

binds methylated DNA (maintains methylation on DNA after replication)

What does DNMT1 do?

add methylation to DNA (maintains methylation on DNA after replication)

What does DNMT3L do?

recruit DNMT3a/b (de novo)

What does DNMT3a/b do?

methylate DNA (de novo)

What does G9a do?

methylate H3K9, recruit HDAC proteins, stimulate DNMT3a/b

What do HDACs do?

Remove acetylation from H3K9 and H3K27

What protein is associated with “changing the status quo”?

G9a

What do TET proteins do?

remove DNA methylation

What do MBD and UHRF1 do?

recruits H3K9-HMT and HDACs when DNA methylation is present

What does H3K4me do?

represses DNMT3L and PRC2

What does H3K36 do?

represses PRC2

H3K9 (methylated vs acetylated vs Ubiquinated/ repression vs activation)

can be methylated/repressed or acetylated/activated

H3K27 (methylated vs acetylated vs Ubiquinated/ repression vs activation)

can be methylated/repressed or acetylatged/activated

H3K4 (methylated vs acetylated vs Ubiquinated/ repression vs activation)

methylated and activation

H3K36 (methylated vs acetylated vs Ubiquinated/ repression vs activation)

methylation and activation

H2AK118/119 (methylated vs acetylated vs Ubiquinated/ repression vs activation)

ubiquinated and repression

DNA (methylated vs acetylated vs Ubiquinated/ repression vs activation)

methylated and repressed

H3K27me3 writer

PRC2

H3K27me3 reader

PRC1, PRC2

H3K27ac writer

CBP

H3K27ac reader

BRD4

H3K9me3 writer

H3K9-HMT, G9a

H3K9me3 reader

HP1

H3K4me writer

TrxG, SET1

H3K4me reader

TrxG

H3K36me writer

TrxG, SET2

H3K36me reader

TrxG

5-methyl C writer

DNMT1, 3a/b

5-methyl C reader

MBP, UHRF1

H2AK119-Ub writer

PRC1