MODULE 1 RESTRICTION ENZYMES

GMO

An organism that may have a

Molecular Cloning meth

Traditional Cloning (CT

Simple PCR

Restriction enzymes

First observed in bacteria in 1952-1972

DNA Ligase

First isolated in 1967

Bacterial Transformation

Established in 1972-73

Cloning Vectors

1970s+. Pieces of DNA that will hold a cloned Gene when transporting the DNA

Polymerase Chain Reaction (PCR)

Kary Mullis, 1983. There are many variations and its pretty much used by everything

Restriction Enzymes

Cuts dsDNA at specific sites. Also cuts phosphodiester bonds between DNA nucleotides. There are different types, Type I and Type III

dsDNA

Phosphodiester

Type I restriction enzyme

Type III Restriction Enzyme