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1
Washed hands or used alcohol based hand rub for hand hygeine.
2
applied non latex exam gloves.
3
assembled equipment and supplies.
4
placed approximately 2-5 g of feces in a paper cup.
5
added 30 mL of flotation solution
6
using the tongue depressor, mix the feces to produce an evenly suspended emulsion.
7
poured the suspension through a 10 cm gauze square into another cup.
8
filled the shell vial with the filtered solution to form a convex dome at the rim.
9
placed a cover slip on top of the meniscus.
10
verbalized leaving the cover slip in place for 10-20 minutes.
11
picked the cover slip straight up and placed on a glass slide, fluid side down.
12
removed non latex gloves and washed hands or used alcohol based hand-rub for hand hygeine.