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scales pros
easily removed with minimal risk, traditional structure
scales cons
not all fish have scales, vary between location on body for different species, can be regenerated, must look at 5-10 scales per fish
rays, spines, bones, lenses pros
rays/spines can be removed without killing fish, less likely to be lost/regenerated, most fish have rays, spines, bones, or eye lenses
rays, spines, bones, lenses cons
bones/lens removal kills fish, may not have good agreement with scales, bony material may be resorbed
otolith pros
very fine resolution of growth, not resorbed, lost or regenerated
otolith cons
removal is always lethal