Bloodbanking Proficiency 2

When is Weak D reactive

AHG

Demographics for Weak D testing

donors and pregnancy

Examples of IAT

weak d, ab screen, ab panel, crossmatch

AHG reagents

poly ahg, anti-IgG ahg, anti-C3d ahg, CCC

Principle of antibody screen

test using patient serum against reagent cells for high frequency antibodies

How many screen cells for antibody screen

2-3

How many panel cells for antibody panel

8-20

Rule of three

the antigen positive cells react, three antigen negative cells don’t

Purpose of selected cells

rule in cells that don’t follow rule of three

Follow up for positive antibody panel

phenotype, honor IgG ab when crossmatching

Crossmatch principle

test using patient serum against donor red cells for compatibility

Source of cell suspension for crossmatch

donor segment

Results for antibody screen

pos or neg

Results for crossmatch

compatible or incompatible

When to stop crossmatch

if agglutination occurs at any phase

When to use abbreviated crossmatch

5 year history is negative, current screen is negative

When to use complete crossmatch

positive history, no known history, current positive screen