Week 5/6 Quiz BOTH Attempts Combed

Immunohematology II

An antibody shows strong reactions in all phases of testing (IS, 37, and AHG) with all panel cells coming up positive.  The patient's serum is then tested with cord blood (from an infant's umbilical cord) and the reaction is negative.  What antibody is suspected?

Anti-I

The pattern of reactivity on an antibody identification panel suggests the presence of anti-E.  One of the E-positive cells producing a positive reaction in AHG is also positive for the V antigen, but the rest of the panel cells are negative for the V antigen.  What should the lab professional do about the V antigen?

Rule it out because the positive reaction in the V positive cell can be explained by the presence of the more plausible antigen, E.

The purpose of the autocontrol is to:

detect the presence of autoantibodies

Which of the following is NOT a low frequency antigen?

Le^a

You have performed an antibody panel and have presumptively identified Anti-E.  However, you only have one heterozygous cross-off for K.  How should you proceed?

Find a cell from another panel that is positive for K and negative for E to run through AHG.

In the process of identifying an antibody, the laboratory professional observed 2+ reactions with 3 out of the 11 panel cells in the immediate spin phase.  This reactivity disappeared at 37 degrees and in AHG, so that all 11 panel cells were negative at AHG.  The antibody is most likely:

Anti-N

You've confirmed that you have Anti-Jk^b in the patient's serum.  The antigen C^w is present on a cell that does NOT have Jk^b on it, and there is an unexplained positive reaction in AHG.  How do you proceed?

Consider Anti-C^w as a possible 2nd antibody, and take steps to confirm.

If anti-c (little c) has been tentatively identified in a patient's serum, but anti-E, anti-K, and anti-S have not been fully excluded, RBCs of what phenotypes would need to be included as selected cells?

c neg, E+, K+, S+

All of the following are considered low frequency antigens, except:

Js^b

A female patient is due to have elective surgery and has an ABO/Rh typing and antibody screen performed in case she needs blood transfused during surgery.  She has no history of pregnancy or prior transfusions, but her antibody screen comes back positive!  Of the options below, given the patient's history, which antibody is the most likely culprit?

Anti-Le^a

The ABO Type of all antibody identification panel cells is:

O

A CLS is working up a patient for whom several units of blood have been requested prior to surgery.  The patient's antibody screen is negative.  The CLS proceeds with crossmatching each of the units, and one unit came up incompatible (3+ reaction) at AHG.  Which of the following antibodies may be the cause of the incompatibility?

Anti-Kp^a

Which of the following phenotypes will get a heterozygous cross-off for the 'f' antigen?

R₁r

If anti-s has been preliminarily identified in a patient's plasma, but anti-K, anti-E, and anti-c still need one more cross-off each, RBCs of what phenotypes would need to be included as selected cells?

s-negative, K-positive, E-positive, c-positive

You suspect you have an anti-Fy^a in the patient's serum.  The reactions in AHG are 3+ with some Fy^a-positive cells, and 1+ with other Fy^a-positive cells.  Why might that be?

Anti-Fy^a is known for exhibiting dosage.

Which of the following tasks is a component of final confirmation in antibody identification?

Performing antigen-typing on the patient and they are negative for the antigen to which they've produced an antibody.

You've performed all your cross-offs on an antibody panel, and are working on rule-outs now.  You come to Le^a and see one X in its column.  How do you proceed?

Put a '1' above it; need to get 1 more cross-off using selected cells.

In the process of identifying an antibody, the laboratory professional observed 2+ reactions with 3 out of the 11 panel cells in the immediate spin phase.  This reactivity disappeared at 37 degrees and in AHG, so that all 11 panel cells were negative at AHG.  The antibody is most likely:

Anti-N

You have performed an antibody panel and have presumptively identified Anti-E.  However, you only have one heterozygous cross-off for K.  How should you proceed?

Find a cell from another panel that is positive for K and negative for E to run through AHG.

You've confirmed that you have Anti-Jk^b in the patient's serum.  The antigen C^w is present on a cell that does NOT have Jk^b on it, and there is an unexplained positive reaction in AHG.  How do you proceed?

Consider Anti-C^w as a possible 2nd antibody, and take steps to confirm.